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Integration of Multiple Gene Copies

Wang, J. J., Rojanatavorn, K., and Shih, J. C. H. 2004. Increased production of Bacillus keratinase by chromosomal integration of multiple copies of the her A gene. Biotech. Bioeng., 87,459-464. [Pg.238]

Figure 7. Stepwise integration of multiple copies of the cloned multiple genes into the host chromosome(s), 259A(LNH-ST)(Int 0) (shown in A) contains fewer copies of the integrated xylose-metabolic genes than 259A(LNH-ST)(Int 3) (shown in B)... Figure 7. Stepwise integration of multiple copies of the cloned multiple genes into the host chromosome(s), 259A(LNH-ST)(Int 0) (shown in A) contains fewer copies of the integrated xylose-metabolic genes than 259A(LNH-ST)(Int 3) (shown in B)...
Our unique gene-integration technique that can effectively integrate multiple copies of multiple genes into the yeast chromosome has made it possible for us to develop (the perfect) "stable" genetically engineered yeasts that can effectively... [Pg.157]

Kiel, J.A. et al. (1995) A general method for the consecutive integration of single copies of a heterologous gene at multiple locations in the Bacillus subtilis chromosome by replacement recombination. Appl Environ. Microbiol, 61 (12), 4244-4250. [Pg.286]

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Copy

Copying

Genes multiple

Integrals multiple

Integrator gene

Multiple copy

Multiple integration

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