Insulin immobilized system

Bioluminescence can also be used as the basis for immunoassay. For example, bacterial luciferase has been used in a co-immobilized system to detect and quantify progesterone using a competitive immunoassay format (34), and other luciferase-based immunoassays have been used to quantify insulin, digoxin, biotin, and other clinically important analytes (35). 

Immobilization of glucose oxidase via carbodiimide chemistry onto a poly(acrylic acid) grafted porous cellulose substrate was performed by Ito, et al. (14). They showed that this system s permeability to insulin in response to a 3600 mg% glucose challenge was 1.7 times greater than the permeability prior to the addition of glucose. 

Immobilized metal-affinity chromatography Na+-dependent alanine-insensitive proline uptake system (SLC6A20) Integrin-mobilferrin pathway membrane protein system involved in the transport of ferric iron also inosine-5 - monophosphate Inducible oxide synthetase Iifitiator element Inositol 1,4,5-triphosphate Immobilized pH gradient Isopropylthio-p-D-galactosidase Isopropylthio-p-D-galactopyranoside Inverted repeat insulin receptor... 

Of course, insulin release is not the unique target in the dehvery field. Indeed, for example, Ishihara [47] matches the problem of urea release using a non-erodible membrane. The system is constituted by a pH-sensitive membrane (copolymerization of 4-carboxyacrylanilide and methacrylate) sandwiched among a membrane containing urease immobilized in free radically cross-linked A, A -methylenebisacrylamide. System permeability with respect to a model dmg ((1,4-bis-(2-hydroxyethoxy)benzene) varies with urea concentration in the external environment. 

Immobilized islets of Langerhans are able to respond to external glucose concentrations and release insulin into the systemic circulation. A number of animal studies have shown that alginate-polylysine encapsulated pancreatic islets were successful in correcting the diabetic state in rats for periods of two to three weeks. 

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