Insulin biotinylation

An increased epithelial permeability of hydrophilic compounds, that is terbutaline (Mw 225 Da), 99mTc-labelled diethylene triamine penta-acetate (99mTc-DTPA Mw 492 Da), 113mln-labelled biotinylated DTPA (Mw 1,215 Da) and insulin, has been demonstrated in smokers as compared to non-smokers [8, 62, 84, 127, 159],... 

Imniunoaffinity separations performed on a matrix-assisted laser desorption ionization-mass spectrometer (MALDI-MS) probe tip have given rise to the term PAMS (probe affinity MS) [134]. Antibiotin covalently bound to the surface of the probe allowed MS discrimination of bovine insulin with different degrees of biotinylation. The method has also been used to detect lysozyme in human tears by binding antilysozyme to the MS probe. Based on the same PAMS principle, modifications aimed to obtain faster and improved retention of Abs have been carried out by immobilizing the Abs on the probe via a nitrocellulose film instead of binding them directly to the MALDI probe surface [135]. The procedure allowed determination of a therapeutic peptide and one metabolite. 

Davis ME, et al. Local myocardial insulin-like growth factor 1 (IGF-1) delivery with biotinylated peptide nanofibers improves cell therapy for myocardial infarction. Proc Natl Acad Sci USA 2006 103 8155-60. 

Padin-lruegas, M. E., Misao, Y., Davis, M. E., Segers, V. F., Esposito, G., Tokunou, T., Urbanek, K. et al. 2009. Cardiac progenitor cells and biotinylated insulin-Uke growth factor-1 nanofibers improve endogenous and exogenous myocardial regeneration after infarction. Circulation, 120,876-887. 

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