Infrared multi-photon dissociation

Goolsby, B.J. Brodbelt, J.S. Analysis of protonated and alkali metal cationized aminoglycoside antibiotics by collision-activated dissociation and infrared multi-photon dissociation in the quadrupole ion trap. J. Mass Spectrom., 35,1011-1024 (2000). 

In all three instrumental configurations, it is possible to induce decomposition of the ion trapped inside the ICR cell not only by collisional experiments, but also by interaction with slow electrons (electron capture dissociation, ECD) or by irradiation with an infrared (IR) laser beam (infrared multi photon dissociations, IRMPD). Experiments of this type (mainly devoted to polypeptide identification) allow to maintain the high-vacuum conditions inside the ICR cell, which are necessary to achieve the high-resolution conditions. 

M. C. Crowe and J. S. Brodbelt, Infrared multi-photon dissociation (IRMPD) and collisionally activated dissociation of peptides in a quadrupole ion trap (QIT) with selective IRMPD of phosphopeptides, J. Am. Soc. Mass Spectrom. 15, 1581-1592, (2004). 

J. W. Flora and D. C. Muddiman, Selective, sensitive, and rapid phosphopeptide identification in enzymatic digests using ESI-FTICR-MS with infrared multi-photon dissociation. Anal. Chem. 73, 3305-3311 (2001). 

Abstract In this chapter we review recent advances in theoretical methods to understand and rationalize anharmonic vibrational spectroscopy (IR-MPD and IR-PD) and collision induced dissociations (CID) in the gas phase. We focused our attention on the application of molecular dynamics-based methods. DFT-based molecular dynamics was shown to be able to reproduce InfraRed Multi-Photon Dissociation (IR-MPD) and InfraRed Pre-Dissociation (IR-PD) action spectroscopy experiments, and help assign the vibrational bands, taking into account finite temperature, conformational dynamics, and various anharmonicities. Crucial examples of dynamical vibrational spectroscopy are given on the protonated AlanH" series (related to IR-MPD in the 800-4,0(X) cm domain), ionic clusters (related to IR-PD in the 3,000-4,(XX) cm region), and neutral peptides (related to IR-MPD in the far-lR). We give examples from simple (e.g., cationized urea) to more complex (e.g., peptides and carbohydrates) molecular systems where molecular dynamics was particularly suited to understanding CID experiments. 

Hughes, R.J. March, R.E. Young, A.B. Multi-photon dissociation of ions derived from 2-propanoI in a QUISTOR with low-power cw infrared-laser radiation. Int. J. Mass Spectrom. Ion Processes. 1982,42, 255-263. 

Bomse, D. S. Woodin, R. L. Beauchamp, J. L. Molecular activation with low-intensity Cw infrared-laser radiation—Multi-photon dissociation of ions... 

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