Influence of sample preparation

Pridmore, A.M., Devine, D.A., Bonass, W.A. and Silley, P. (1999) Influence of sample preparation technique on two-dimensional gel electrophoresis of proteins from Porphyromonas gingivalis. Letters in Applied Microbiology 28, 245-249. 

Reduced influence of sample preparation (a single sectioning of the original material). 

Menzies, N. W., and Bell, L. C. (1988). Evaluation of the influence of sample preparation and extraction technique on soil solution composition. Aust.J. Soil Res. 26(3), 451—464. 

Luthria, D.L. Mukhopadhyay, S. 2006. Influence of sample preparation on assay of phenolic acids from eggplant. J. Agric. Food Chem. 54 41-47. 

Figure 2. Influence of sample preparation time on apparent heterogeneity. pMAB and hAB-1 were mixed with non-reducing SDS-PAGE sample buffer and boiled for various times prior to electrophoresis. Boiling times Lanes 1,2 1 minute, lanes 3,4 5 minutes and lanes 5,6 10 minutes. Samples pMAB (lanes 1,3 and 5), hAB-1 (lanes 2,4 and 6).

A systematic study of the influence of sample preparation on the differential thermal analysis and thermogravimetric behaviour was then undertaken. The results were shown to be very sensitive to the method of polymerization and residual traces of catalyst. Initiation of cyclization at... 

Instrumental detection limits should be avoided, since they do not Include the influences of sample preparation, cleanup, etc. on the detection limit. In this regard, detection limits based on signal to noise ratio should also be avoided. If used, they should be obtained from a complete analytical procedure and not Just the Instrument. 

The above facts do not favour protein or peptide quantitation using MALDI-MS. Some problems are associated with MALDI-MS quantification i) low shot-to-shot reproducibility, ii) various signal suppression effects, and iii) strong influence of sample preparation and matrix crystallisation. Nevertheless, it is possible to use MALDI-MS to obtain absolute or relative quantitation. In most cases, the idea is to use an internal standard for an absolute quantitation, but this standard must have the same physico-chemical characteristics as the quantified peptide. The use of a different peptide in terms of sequence may result in different desorption and ionisation properties. Usually, the internal standard is the same peptide labelled with a stable isotope to modify slightly the mass. 

Pure COP and partially crystalline blends with less than 80 mass% PC are rather brittle and show a consistently low impact resistance. Blends of this composition consist of a PC phase partially miscible with COP (Figure 9.25). The change in morphology is responsible for the sudden improvement of the impact resistance qualities in blends with more than 80 mass% PC. These particular products are almost amorphous and consist of dispersed COP spheres embedded in a tough PC matrix. The morphological and mechanical studies on COP + PC blends demonstrate the strong influence of sample preparation and blend... 

Kochana, J., Wilamowski, J. and Parczewski, A. TLC profiling of impurities of l-(3, 4-methylenedioxyphenyl)-2-nitropropene an intermediate in MDMA synthesis. Influence of sample preparation methods and conditions. J. Liq. Chromatogr. Relat. Technol. 27 2463-2470, 2004. 

Table 1 Influence of sample preparation on detection limits in TXRF (after Klockenkamper R (1997))...

Rogez D, Francius G, Finkelmann H, Martinoty P (2006a) Shear mechanical anisotropy of side chain liquid crystal elastomers influence of sample preparation. Eur Phys J E 20 369-378 Rogez D, Brandt H, Finkelmann H, Martinoty P (2006b) Shear mechanical properties of main chain liquid crystalline elastomers. Macromol Chem Phys 207 735-745... 

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