Inertsil columns

Closely clustering objects, such as the three Inertsil columns, behave very similarly. 

Detection of metabolites derived from Trp-P-1 during primary culture was performed by the method described by Minamoto and Kanazawa (i 7) with some modifications. Briefly, intracellular metabolites and Trp-P-1 were extracted with ethyl acetate firom these cells. The metabolites were detected using a HPLC (Hitachi L-7100) equipped with a UV detector (Hitachi L-7420) and electrochemical detector (ECD IRJCA E 875) connected in series. An Inertsil column, ODS (i.d. 4.6 x 150 mm), was maintained at 35 C. The mobile phase... 

Column L-Column LiChrospher-C18 HRC-ODS Inertsil ODS L-Column Zorbax SB-C8... 

Precolumn Hitachigel 3011-0 (100mmx4.6mm, 5pm), analytical column Inertsil ODS-5 (150mmx4.6mm, 5pm) C18... 

Figure 6.2 A comparison of HPLC separation methods, (a) HPLC of a-chaconine and a-solanine in the flesh and the peel of one variety of potato. Conditions column, Inertsil NH2 (5 xm, 4.0 X 250 mm) mobile phase, acetonitile/20 mM KH2PO4 (80 20, v/v) flow rate, I.OmL/min column temperature, 20°C UV detector, 208 nm sample size, 20 (xL. (b) HPLC chromatogram of approximately 1 xg of each of potato glycoalkaloids and their hydrolysis products 1, solasonine (internal standard) 2, a-solanine 3, a-chaconine 4, P2-solanine 5, pi-chaconine 6, (32-chaconine 7, y-solanine 8, y-chaconine. Conditions column. Resolve Cl 8 (5 (xm, 3.9 x 300 mm) mobile phase, 35% acetonitrile/100 mM ammonium phosphate (monobasic) at pH 3 flowrate, I.OmL/min column temperature, ambient UV detector, 200 nm sample size, (c) HPLC chromatogram of the aglycones solanidine and solasodine. Conditions column Supelcosil C18-DB (3 (xm, 4.6x150 mm) mobile phase, 60% acetonitrile/10 mM ammonium phosphate pH 2.5 flowrate, 1.0 mL/min column temperature, ambient UV detector, 200 nm.

A-11 equipped with a model 655-40 autosampler and a UV detector (Hitachi model 655A UV monitor) set at 208 nm. Column temperature was controlled with a Coolnics model CTR-120 device (Komatsu Electronics, Tokyo, Japan). Chromatogram peak areas were integrated with a Hitachi D-2500 chromato-integrator. The column was an Inertsil NH2 (5 p,M, 4.0 x 250 mm)... 

Figure 6.6 HPLC chromatogram of the extract from Superior potato flesh (a) and of the same extract spiked with standards (b). Identification p.1, chlorogenic acid p.2, chlorogenic acid isomer p.3, caffeic acid p.4, p-coumaric acid p.5, ferulic acid p.6, t-cinnamic acid. Column, Inertsil ODS-3 V (5 p.m, 4.0 X 250 mm) flow rate, l.OmL/min column temperatures, 20°C mobile phase, acetonitrile 0.5% formic acid (gradient mode) detector, UV at 280 nm.

Figure 6.7 LC-MS chromatograms of an extract of Superior potato peel monitored at 280 nm, 340 nm, and TIC. Column Inertsil ODS-3 (3 p,m, 4.0 x 150 mm). Flow rate 0.2 mL/min. Column temperature 30°C. Mobile phase acetonitrile 0.5% formic acid (gradient mode).

Liquid chromatography/mass spectrometry analyses were performed with an ion trap mass spectrometer (LCQ, Thermo Fisher Scientific Inc., MA) equipped with an HPLC system (Agilent, CA Model 1100) connected with a diode-array detector (DAD, G1315A). The sample solution (1-5 p,L) was applied on an Inertsil ODS-3 column (2.1 x 150 mm, 3 p,m, GL... 

HCIO4 extn, SPE cleanup, 1-hexanesulfonic acid addn, online trace enrichment on Inertsil Cg, 5 m, preconcn column and switching to analytical column... 

Nakazato et al. (284) determined the CF and geniposidic acid content in leaves of Eucom-mia ulmoides used in health foods and beverages. The samples (dried leaves or granules) were refluxed with water, filtered, acidified, and cleaned up on aMegaBondElut Cl8 cartridge linked to an Elut SAX cartridge. The eluate (with MeOH-Tris hydrochloride buffer) was applied to a Inertsil ODS-2 column at 40°C and eluted with an ACN-phosphate solution mixture. Detection was performed at 240 nm, reaching a DL of 10 fig/g. 

Sakamoto et al. (332) applied an HPLC method to analyze capsaicinoids and their phenolic intermediates in Capsicum annuum and to characterize their biosynthetic status. Descended fruit is extracted with 80% EtOH, the extract is evaporated to dryness, and the residue is dissolved in DMSO and analyzed on an Inertsil ODS-2 column with gradient elution (ACN-aq. TFA) and detection at 280 nm. The method was used to study changes in capsaicin content during ripening. 

The optimum separation conditions for 11 CPs of DCPs and TCPs were examined in an isocratic elution. The separation column used was oct-adecyl silica, Inertsil ODS-3, which was provided from GL Sciences Inc. (Tokyo, Japan). Figure 11.11 indicates the relationship of capacity factor, k, and pH for different organic solvents in (A) 50% acetonitrile buffer and (B) 60% methanol buffer. The value of k represents alternatively the elution time. In the case of acetonitrile solvent shown in Fig. 11.11A, the separation of the selected 11 CPs was not observed completely in any pH region. On the contrary, the mobile phase of the 60% methanol buffer... 

Fig. 11.11. Relationship of the capacity factor with pH in (A) 50% acetonitrile buffer and (B) 60% methanol buffer. An Inertsil ODS-3 column was used in an isocratic elution.

Fig. 11.12. Schematic diagram of a column-switching HPLC system. V-l, V-2, and V-3 switching valves. The position of °—c means position 1, and ° ° is position 2. P-1 and P-2 pumping system at 0.2 mL min-1 flow rate. Detector electrochemical detector with diamond electrodes. Column-1 and Column-2 Inertsil ODS-3. Loop 500 pL. A Mobile phase of 60% methanol-water containing 0.5% phosphoric acid.

Shimizu et al. [75] described a column-switching HPLC method for the simultaneous determination of omeprazole and its two main metabolites, 5-hydroxyomeprazole and omeprazole sulfone, in human plasma. Omeprazole and its two metabolites and lansoprazole as an internal standard were extracted from 1 ml of alkalinized plasma sample using diethyl ether-dichloromethane (45 55). The extract was injected into a column I (TSK-PW precolumn, 10 /im, 35 mm x 4.6 mm) for cleanup and column II (Inertsil ODS-80A column, 5 /an, 150 mm x 4.6 mm) for separation. The mobile phase consisted of phosphate buffer-acetonitrile (92 8, pH 7) for cleanup and phosphate buffer-acetonitrile-methanol (65 30 5, pH 6.5) for separation, respectively. The peak was detected with a UV detector set at a wavelength of 302 nm, and total time for chromatographic separation was approximately 25 min. The validated concentration ranges of this method were 3-2000 ng/ml for omeprazole, 3-50 ng/ml for 5-hydroxyomeprazole, and 3-1000 ng/ml for omeprazole sulfone. Mean recoveries were 84.3% for omeprazole, 64.3% for 5-hydroxyomeprazole, and 86.1%... 

Column Inertsil ODS2 column (Chrompack) Mobile phase 50 mM ammonium acetate buffer (pH 5.0) and... 

An atmospheric pressure chemical ionization (APCI-LC-MS) (Sciex API 150 EX) method was developed for the determination of zaleplon and zolpidem in the whole blood. After single-step LLE, the hypnotics were separated by gradient-elution with an ammonium formate buffer/acetonitrile eluent on an Inertsil ODS-3 column. Methaqualone was used as IS. The recovery was higher than 70% for both hypnotics and the IS. The method was successfully applied to forensic cases [11]. 

Inertsil ODS-3 column Ammonium formate buffer-acetomitrile Mass spectrometry Determination in whole blood applied to forensic cases [11]... 

Holtzapple et al. developed an immunoassay method for determination of four fluoroquinolone compounds (including ciprofloxacin) in liver extracts [64]. In this method, an immunoaffinity capture SPE column was used, that contained anti-sarafloxacin antibodies covalently cross-linked to protein G. After interfering liver matrix compounds had been washed away, the bound ciprofloxacin was eluted directly onto the HPLC column. The HPLC system used a 5 pm Inertsil phenyl column (15 cm x 4.6 mm i.d.), with 0.1 M-glycine hydrochloride/acetonitrile (17 3) as the mobile phase (eluted at a rate of 0.7 mL/min). Fluorimetric detection at 444 nm was used after excitation at 280 nm. The recovery of ciprofloxacin ranged from 85.7 to 93.5%, and the detection limit was... 

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