INDEX separation from drug

Quitasol and Krastins [504] used a polyhydroxyethylmethacrylate (Universal Anion, Grace Alltech, USA) as a stationary phase 5 mmol/L potassium nitrate adjusted to pH 3.5 with nitric acid serving as an eluent. Under these chromatographic conditions, the organic phosphate can be separated to baseline from orthophosphate and orthophosphite within 10 min. Because Quitasol and Krastins determined only the drug component in various pharmaceutical formulations, they used a refractive index detector for the sake of simplicity. This procedure can be validated in terms of precision, accuracy, and linearity without any difficulties. 

GC is applied in a variety of ways and is one of the most important separation techniques in this particular area. GC provides the retention time or retention index (RI) of an unknown substance that can be used for its identification. GC is routinely utilized to separate the analyte from endogenous interferences for more specific identification via mass spectrometry and can also be used to provide quantitative information about the drugs present. The following applications focus on the identification and quantification of drugs and volatiles in biological fluids by GC. 

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