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Immunostaining Enhancement

Holgate, C., Jackson, P., Cowen, P., and Bird, C. (1983) Immunogold-silver staining new method of immunostaining with enhanced sensitivity./. Histochem. Cytochem. 31, 938. [Pg.1074]

Kitamoto, T., Ogomori, K., Tateishi, J., and Prusiner, S. (1987) Formic acid pretreatment enhances immunostaining of cerebral and systemic amyloids. Lab. Invest. 57, 230-236. [Pg.84]

Krenacs, T., Stiller, D., Krenacs, L., Bahn, H., Molnr, F., and Dux, L. (1990) Sarcoplasmic reticulum (SR) Ca +-ATPase as a marker of muscle-cell differentiation immunohistochemical investigations of rhabdomyosarcomas and enhancement of immunostaining after sodium methoxide treatment. Acta Histochem. 88, 159-166. [Pg.92]

Appropriate controls should always be run with any immunocytochemical procedure. Controls may include omitting the primary antibody, substituting preimmune serum, normal serum, or normal IgG for the primary antibody, adsorbing the primary antibody against the antigen, or immunostaining with an unrelated antibody. To enhance preservation, 3-5% purified sucrose and 0.5 mM calcium chloride may be added to the buffer. [Pg.353]

Rapid immunohistochemical study of frozen sections is necessary for intraoperative diagnosis in some cases. Rapid immunostaining is also helpful in confirming or excluding tumor clearance in resection margins or in detecting micrometastases in sentinel lymph nodes in breast cancer patients. Two methods to immunostain frozen sections are the enhanced polymer one-step staining (EPOS) system and the EnVision system both systems are detailed later. [Pg.138]

MICROWAVE HEAT-ENHANCED DOUBLE IMMUNOSTAINING OF NUCLEAR AND CYTOPLASMIC ANTIGENS... [Pg.183]

Endogenous peroxidase activity is quenched by treatment with 0.5% H202 in methanol, immunostaining is carried out using a Vectastain Elite ABC kit, and visualization of the secondary antibody is achieved with DAB enhanced with nickel (Vector Laboratories). [Pg.192]

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