Immunoproteomics

The advent of immunoproteomics made possible the identification of highly immunogenic proteins that can be used for vaccine development. Proteins that have the greatest potential for eliciting a protective immune response are collectively referred to as the pathogen s immunome. Immunoproteomics has been utilized to characterize the immu-nome of B. anthracis for the development of a safer and equally efficacious vaccine. The immunoreactive proteins are first identified by using 2DE Western blot analysis in conjunction with mass spectrometry. In B. anthracis, for example, antisera from humans post-infected with anthrax were used to probe Western blots of its various... 

T., and Jungblut, P.R. 2002, Immunoproteomics of Helicobacter pylori infection and relation to gastric disease. Proteomics 2 313-324. 

Immunoproteomics, i.e. the comprehensive identification of immunogenic proteins from an organism such as H. pylori, is an attractive approach to determine i) antigens that could be used to increase the sensitivity and specificity of non-invasive diagnostic tools based on serology and ii) vaccine candidates. Here we discuss the current information available on the H. pylori immunoproteome and its potential for application in vaccination or diagnosis. 

The remarkable increase in total reactivity though is in line with the hypothesis that proinflammatoiy alleles of the IL-ip locus were most consistently associated with increased gastric cancer-risk (Gonzalez et al, 2002). Increased inflammation is likely to boost the antibody production which provides a rational for the immunoproteome results. 

U., Jungblut, P. R. and Meyer, T. F. 2002b, A comparison of murine and human immunoproteomes of Helicobacter pylori validates the preclinical murine infection model for antigen screening. Infect.Immun., 70 6494-6498... 

Purcell, A. W. and Gorman, J. J. 2004. Immunoproteomics Mass spectrometry-based methods to study the targets of the immune response. Mol. Cell. Proteomics 3 193-208. 

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