Immunoliposome applications

The "stealth" concept may offer two other opportunities for liposome application (1) Conventional immunoliposomes (see Sec. VI.C) have been shown to be removed rapidly firom the circulation by the MPS (Peeters et al., 1987). The combination of the stealth approach for longer circulation with the attachment of antibodies or antibody fragments may provide a means of delivery of drugs to their sites of action with a high degree of specificity. This could be useful for treating leukemia, graft-vs.-host diseases, and HIV disease. 

Mastrobattista E et al (2002) Functional characterization of an endosome-disruptive peptide and its application in cytosolic delivery of immunoliposome-entrapped proteins. J Biol Chem 277 27135-27143... 

Mori A, Huang L (1993) Immunoliposome targeting in a mouse model optimization and therapeutic application. In Gregoratos G (ed) Liposome technology, vol 3. CRC Press, Boca Raton, FL, pp 153-163... 

Fig. 7.1 Detection procedure a Immobilization of hemagglutinin (or antigen peptide) on an Au electrode, b Binding of immunoliposome with hemagglutinin onto the Au electrode through competitive antigen-antibody reaction, c Destruction of immunoliposome by addition of ethanol, d Adsorption of Ru(II) complex by heating at 60 °C for 10 min. e ECL measurement on application of potential. Reprinted with permission from Ref. [21]. Copyright 2008 American Chemical Society...

Yanagie, H., Tomita, T., Kobayashi, H. et al. 1991. Application of boronated anti-CEA immunoliposome to tumour cell growth inhibition in in vitro boron neutron capture therapy model. Br. J. Cancer 63 522-6. 

Target-sensitive immunoliposomes are designed to destabilize upon binding to the target cell and to release their contents in high concentrations at the cell surface. Target-sensitive delivery could be applicable to a wide variety of target cells but is limited to small molecules, which are rapidly transported into the cells, and to macromolecules, which can bind to cell surface receptors. 

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