Peptide immunogen preparation

Friede, M., Van Regenmortel, M.H.V., and Schuber, F. (1993) Lyophilized liposomes as shelf items for the preparation of immunogenic liposome-peptide conjugates. Anal. Biochem. 211, 117-122. 

Add the solution prepared in step 2 to the protein solution to obtain at least a 10-fold molar excess of small molecule to protein. In the case of the peptide-protein immunogen conjugate, add the 500 pi of peptide solution to the 200 pi of protein solution. 

Iwai, K., Fukuoka, S.-I., Fushiki, T., Kido, K., Sengoku, Y., and Semba, T. (1988) Preparation of a verifiable peptide-protein immunogen direction-controlled conjugation of a synthetic fragment of the monitor peptide with myoglobin and application for sequence analysis. Anal. Biochem. 171, 277-282. 

In preparation for use as a surrogate vaccine, these peptides were synthesized as conjugates to tetanus toxoid, and in this form also competed with the 0-antigen polysaccharide for binding [104]. Whether these peptides will function as effective immunogenic mimics will likely depend on their propensity to adopt the bioactive conformation in solution. A certain amount... 

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