Immunofluorescence microscopes

Remarkably, Brassica napus pollen was reported to have a 22 kDa cutinase that cross-reacted with antibodies prepared against F. solani f. pisi cutinase [134]. Although a 22 kDa and a 42 kDa protein that catalyzed hydrolysis of p-nitrophenyl butyrate were found in this pollen, only the former catalyzed cutin hydrolysis. Immunofluorescence microscopic examination suggested that the 22 kDa protein was located in the intine. Since the nature of the catalytic mechanism of this enzyme has not been elucidated, it is not clear whether this represents a serine hydrolase indicating that plants may have serine and thiol cutinases. The role of the pollen enzyme in controlling compatibility remains to be established. 

Shaw, G., Osborn, M., and Weber, K., An immunofluorescence microscopical study of neurofilament triplet proteins, vimentin and glial fibrillary acidic protein within the adult rat brain, Eur. J. Cell Biol., 26, 68, 1981. 

Miettinen M, Lehto V-P, Virtanen 1. Immunofluorescence microscopic evaluation of the intermediate filament expression of the adrenal cortex and medulla and their tumors. Am J Pathol. 

Immunofluorescent microscopic examination of fluid expressed from the penile chancre of this patient revealed treponemes. Since he appears to be infected with T pallidu))i, the best course of action would be to (A) Treat with spectinomycin Treat with oral tetracycline Inject intramuscular benzathine penicillin G Give a single oral dose of fosfomycin... 

Immunofluorescence-microscope photograph of a dividing human cell. The chromosomes are coloured blue and the spindle apparatus is in green. 

The recent availability of antibodies to cytoskeletal components offers an additional means to study reovirus interactions with cytoplasmic filaments. Although electron-microscopic techniques can elu-dicate filament structure and distribution, the full extent of cytoplasmic filament organization was not known until indirect immunofluorescence techniques were developed (Weber et al., 1975 Brinkley et al., 1978 Lazarides, 1975). Immunofluorescence microscopic techniques indicate that cytoplasmic filaments form networks within the cell cytoplasm. 

In order to analyze the localization of the aconitase without organelle preparation, we used immunofluorescent microscopic observation. When the cells isolated from etiolated pvunpkin cotyledons were treated with the antibody, weak and small fluorescent signals were observed on the weak fluorescent background throughout whole cells as shown in figure 2. This indicates that aconitase localized in an organelle that represent the small... 

Indirect immunofluorescence assay (IFA) A laboratory test used to detect antibodies in serum or other body fluid. The specific antibodies are labeled with a compound that will make them glow a fluorescent green color when observed microscopically under ultraviolet light. 

Since endocytosis ofLDH was confirmed by TEM images (Figure 13.9), forthe next step, its specific endocytic pathway for membrane entry was determined by immunofluorescence and confocal microscopy. Cells were incubated with LDH-FITC, fixed with 3.7% freshly made formaldehyde, and then stained with either anti-clathrin antibody or anti-caveolin-1 antibody both conjugated to the red fluorescent dye Texas Red (TR). The confocal microscopic images showed that green fluorescent... 

Camargo ME, Ferreira AW. A microscopic immunofluorescence technique with soluble protein antigens fixed to cellulose particles. Int. Arch. Allergy 1970 39 292-300. 

Figure 7. Talin-containing streak-like focal adhesion plaques (A) and beta-actin cytoskeleton (B) in vascular smooth muscle cells in cultures derived from the rat aorta. Immunofluorescence staining, the nuclei couterstained with propidium iodide (A) or Hoechst 33342 (B). Microscope Olympus IX 50, digital camera DP 70, obj. 100, bar=20 pm.

Figure 26. Reconstruction of the tunica intima on the inner surface of a clinically used polyethylene terephtalate vascular prosthesis. A non-modified inner surface of the prosthesis, B immobilization of defined assemblies of protein molecules (e.g., collagenfiarninin or collagen+fibrin) on the inner surface of the graft, C immunofluorescence of von Willebrand factor, a marker of the identity a differentiation of vascular endothelial cells, in human saphenous vein endothelial cells in cultures on the inner surface of a prosthesis coated with collagen and larninin, D detail of a layer of endothelial cells growing on a layer of collagen and fibrin. Note well developed talin-containing focal adhesion plaques. A, B conventional optical microscope, C, D confocal microscope Leica DM 2500 [30,31].

Figure 29. Fiuman osteoblast-like MG 63 cells in cultures on material surfaces modified with carbon nanoparticles. A fullerene Cgo layers deposited on carbon fibre-reinforced carbon composites (CFRC), B fullerene C o layers deposited on microscopic glass coverslips, C terpolymer of polytetrafluoroethylene, polyvinyldifluoride and polypropylene, mixed with 4% of single-wall carbon nanohorns, D the same terpolymer with high crystalline electric arc multi-wall nanotubes, E diamond layer with hierarchically organized micro- and nanostmcture deposited on a Si substrate, F nanocrystalline diamond layer on a Si substrate. Standard control cell culture substrates were represented by a PS culture dish (G) and microscopic glass coverslip (FI). Immunofluorescence staining on day 2 (A) or 3 (B-Fl) after seeding, Olympus epifluorescence microscope IX 50, digital camera DP 70, obj. 20x, bar 100 pm (A, C, D, G,H)or 200 pm (B, E, F) [16].

Double immunofluorescence labeling in conjunction with microwave heating can be used to visualize two markers at the same cellular location in routine formalin-fixed and paraffin-embedded tissue sections (Mason et al 2000). The primary antibodies are either monoclonal antibodies of differing isotype/subclass or antibodies from different species. Labeling is visualized on a conventional fluorescence microscope equipped with a cooled analog monochrome CCD camera (Model C 5985, Hamamatsu Photonics, Billerica, MA) and recorded using off the shelf personal computer hardware and software. Contrary to general belief, paraffin-embedded tissue sections do not show excessive nonspecific fluorescence. 

Minimal change disease is the most common cause of nephrotic syndrome in children, presenting typically with rapid onset of mostly steroid-sensitive nephrotic syndrome, usually with selective proteinuria (albuminuria). Light-microscopic morphology of the kidney is normal and immunofluorescence is negative. Foot process effacement on electron microscopy is the only observed pathology. 

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