Immunoelectron microscopy labeling

Stirling JW, Graff PS (1995) Antigen unmasking for immunoelectron microscopy labeling is improved by treating with sodium ethoxide or sodium metaperiodate, then heating on retrieval medium. J Histochem Cytochem 43 115 123... 

Positions of a few proteins located by immunoelectron microscopy and three positions in the 16S RNA are marked. The puromycin binding site labeled Pm was mistakenly thought to be near the peptidyltransferase center. (B) The 50S subunit. Only a... 

Shimizu, H. and Nishikawa, T. (1993) Application of an image analyser to gold labeling in immunoelectron microscopy to achieve better demonstration and quantitative analysis J Histochem Cytochem 41, 123-128... 

Results from enzymologic studies have suggested that SCCE has an extracellular localization in the stratum corneum.45 This has been corroborated by means of immunoelectron microscopy. With this method SCCE was found intracellularly in association with lamellar bodies in the stratum granulosum. In the transition between the stratum granulosum and the stratum corneum, SCCE is extruded to the extracellular space together with the lamellar bodies. In the stratum corneum specific labeling is found only in the extracellular space, often in association with corneodesmosomes.46... 

One family of IFAPs, the plakins, is responsible for linking IFs with both microtubules and mlcrofllaments. One plakln family member is plectin, a 500,000-MW protein that has been shown to cross-link intermediate filaments with microtubules and actln filaments in vitro. Plectin also Interacts with other cytoskeletal proteins, including spectrin, microtubule-associated proteins, and lamin B. Immunoelectron microscopy reveals gold-labeled antibodies to plectin decorating short, thin connections between microtubules and vimentin. 

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