Immunocytochemistry, cannabinoid

The distribution of cannabinoid receptors has been extensively mapped by quantitative autoradiography, in situ hybridization, and immunocytochemistry. Each of these techniques has its strengths and weaknesses. Properly calibrated, quantitative autoradiography provides the best measure of absolute receptor density. Nonetheless, its spatial resolution is limited and specificity depends on the ligand used. In situ hybridization identifies the cells synthesizing CB i mRNA. However, mRNA levels and protein levels may not necessarily correlate. Immunocytochemistry provides outstanding spatial resolution however, fixation artifacts and unanticipated antibody crossreactivity must be assiduously avoided. For the most... [Pg.300]

Yazulla, S., Studholme, McIntosh, H.H., Ean, S.-H (2000) Cannabinoid receptors on goldfish retinal bipolar cells Electron-microscope immunocytochemistry and whole-cell recordings. Vis. Neurosci. 17, 391 01. [Pg.117]

Key Words Cannabinoid receptors CBi receptor CB2 receptor electron microscopy immunocytochemistry immunoelectron microscopy immunogold immunohistochemistry immunoperoxidase. [Pg.41]