Immunoblotting of Proteins from Single Drosophila Embryos

Immunoblotting of Proteins from Single Drosophila Embryos  

Department of Molecular, Cellular, and Developmental Biology University of Colorado, Boulder Boulder, Colorado 80309-0347 

Grape juice-agar plates and yeast paste for embryo collection (see Sisson, this volume) 

Baskets for washing embryos (these are generally short cylinders of plastic that are sealed at one end with a nylon screen) 

Soft brush for gathering embryos Dissecting microscope Microcentrifuge tubes (1.5 ml) 

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