Immunoassay blood thyroid-stimulating hormone

K3. Kato, N., Ishii, S., Naruse, H., Irie, M., Arakawa, H., and Tsuji, A., Enzyme immunoassay of thyroid-stimulating hormone using dried blood samples. A simple technique of screening for congenital hypothyroidism. Anal. Lett. 13, 1555-1565 (1980). 

Xu, Y.Y., Pettersson, K., Blomberg,K., Hemmila, I., Mikola, H., and Lovgren, T. (1992) Simultaneous quadruple label fluorometric immunoassay of thyroid stimulating hormone, 17 a hydroxyprogesterone, immunoreactive trypsin, and creatine kinase MM isoenzyme in dried blood spots. Clinical Chemistry, 38, 2038 2043. 

Time-resolved approaches for multi-analyte immunoassays have been described recently. Simultaneous determination of LH, follicle stimulating hormone (FSH), hCG, and prolactin (PRL) in a multisite manual strip format has been reported. 88 Four microtiter wells are attached to a plastic strip, two-by-two and back-to-back, such that the wells can be read on a microtiter plate reader. In a quadruple-label format, the simultaneous quantitative determination of four analytes in dried blood spots can be done using europium, samarium, dysprosium, and terbium. 89 In this approach, thyroid-stimulating hormone, 17-a-hydroxyprogesterone, immunoreactive trypsin, and creatine kinase MM (CK-MM) isoenzyme are determined from dried blood samples spotted on filter paper in a microtiter well coated with a mixture of antibodies. Dissociative fluorescence enhancement of the four ions using cofluorescence-based enhancement solutions enables the time-resolved fluorescence of each ion to be measured through four narrow-band interference filters. 

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