Immobilized template

Procedure for Peptide Loop Formation on an Immobilized Template ... 

Yilmaz E, Haupt K, Mosbach K (2000) The use of immobilized templates - A new approach in molecular imprinting. Angew Chem Int ed 39 2115... 

Visnjevski E, Yilmaz E, Bruggemanna O (2004) Catalyzing a cycloaddition with molecularly imprinted polymers obtained via immobilized templates. Appl Catal A 260(2) 169— 174... 

Immobilized nucleic acid bases, nucleosides or oligonucleotides may be used for separation, fractionation and structure determination of various nucleic acids and enzymes participating in their synthesis and degradation. Schott et al. [139,229] made use of immobilized defined oligonucleotides for the selective separation of free nucleotides on the basis of a base-pairing mechanism. Complementary oligonucleotides in the mobile phase are selectively adsorbed on the immobilized template if... 

In other work described in the same publication, a-cyclodextrin was converted to a solid polymer by reaction with epichlorohydrin, and this was used in a reactor to perform the flow reaction of anisole with HOCl. The product was completely pnra-chlorinated, and the reactor could be used repeatedly. Thus, the binding of substrate to an immobilized template has potential for practical synthesis with geometric control of selectivity. 

T 0 conclude this part on dynamic combinatorial chemistry, we will mention the use of a polymer bound N-methyl ammonium ion as a template. The library is generated from the pseudopeptide building block mPro, which gives a dynamic library of hydrazones (Fig. 23). Depending on the conditions, the following will be formed at equilibrium in the absence of a template, 85% of the cyclic dimer and 7% of the cyclic trimer in the presence of the template BTA, 47% of the dimer and 50% of the trimer and in the presence of the immobilized template. 40% of pure trimer can be isolated by washing the resin. 

Researchers have devised a synthesis strategy that entails the use of a sacrificial porous solid support on which the template is immobilized. An example that demonstrates the feasibility of this approach is represented in Fig. 12 [42]. Presently, this new concept is explored further and the imprinting of other immobilized templates has been published [43]. 

Further, imprinting of immobilized templates involves additional synthetic steps and the support material is often destroyed in the process. 

Phadtare, S., Kumar, A., Vinod, V.P., Dash, C., Palaskar, D.V., Rao, M., Shukla, P.G., Sivaram, S., and Sastry, M. (2003). Direct assembly of gold nanoparticle shells on pol3nirethane microsphere cores and their apphcation as enzyme immobilization templates. Chem. Mater. 15 (10), 1944—1949. 

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