Immobilized metal ion

Li, S. and Dass, C., Iron(III)-Immobilized Metal Ion Affinity Chromatography and Mass Spectrometry for the Purification and Characterization of Synthetic Phosphopeptides, Anal. Biochem., 270, 9, 1999. 

Amini, A., Chakraborty, A., Regnier, F.E. (2002). Simplification of complex tryptic digests for capillary electrophoresis by affinity selection of histidine-containing peptides with immobilized metal ion affinity chromatography. J. Chromatogr. B 772, 35-44. 

Metal-chelate affinity chromatography is a powerful purification technique whereby proteins or other molecules can be separated based upon their ability to form coordination complexes with immobilized metal ions (Porath et al., 1975 Lonnerdal and Keen, 1982 Porath and Belew, 1983 Porath and Olin, 1983 Sulkowski, 1985 Kagedal, 1989). The metal ions are stabilized on a matrix through the use of chelating compounds which usually have multivalent points of interaction with the metal atoms. To form useful affinity supports, these metal ion complexes must have some free or weakly associated and exchangeable coordination sites. These exchangeable sites then can form complexes with coordination sites on proteins or other molecules. Substances that are able to interact with the immobilized metals will bind and be retained on... 

Porath, J. (1992) Immobilized metal ion affinity chromatography. Protein Expr. Purif. 3, 263-281. 

Porath, J., and Olin, B. (1983) Immobilized metal ion affinity adsorption and immobilized metal ion affinity chromatography of biomaterials. Serum protein affinities for gel-immobilized iron and nickel ions. Biochemistry 22, 1621-1630. 

Zhou, W., Merrick, B.A., Khaledi, M.G., and Tomer, K.B. (2000) Detection and sequencing of phos-phopeptides affinity bound to immobilized metal ion beads by matrix-assisted laser desorption/ioniza-tion mass spectrometry./. Am. Soc. Mass. Spectrom. 11(4), 273-282. 

Figure 6.17 Schematic representation of the basic principles of metal chelate affinity chromatography. Certain proteins are retained on the column via the formation of coordinate bonds with the immobilized metal ion (a). The actual structure of the most commonly used metal chelator, iminodiacetic acid, is presented in (b)...

Cao P. and Stults J.T. (1999), Phosphopeptide analysis by on-line immobilized metal-ion affinity chromatography-capillary electrophoresis-electrospray ionization mass spectrometry, J. Chromatogr. 853(1-2), 225-235. 

IMAP (immobilized metal ion affinity-based fluorescence polarization) Molecular Devices Trivalent metal ion containing beads bind to phosphorylated peptide producing FP signal change... 

Todorova, D. and Vijayalakshmi, M. A., Immobilized metal-ion affinity chromatography, in Handbook of Affinity Chromatography, 2nd edn., Hage, D.S., Ed., CRC Press, Boca Raton, FL, 2005, Chap. 10. 

Chaga, G.S., Twenty-five years of immobilized metal ion affinity chromatography Past, present and future, J. Biochem. Biophys. Methods, 49, 313-334, 2001. 

Metal-chelate affinity chromatography (Immobilized-metal (Ion) affinity chromatography)... 

In 1970s, first application of metal-chelate affinity chromatography which is later named as "immobilized-metal (ion) affinity chromatography (IMAC) was perfomed. Metal-chelate chromatography technique exploits selective interactions and affinity between transition metal immobilized on a solid support (resin) via a metal chelator and amino acid residues which act as electron donors in the protein of interest [25-26]. As well as aromatic and heterocyclic compounds, proteins such as histidine, tyrosine, tyriptophane and phenylalanine posses affinity to transition metals which form complexes with compounds rich in electrons [25,27]. 

R. Woker, B. Champluvier, M. R. Kula, Purification of S-oxynitrilase from Sorghum hicolor by immobilized metal ion affinity chromatography on different carrier materials./. Chromatogr. 1992, 584, 85-92. 

The technique immobilized metal ion affinity chromatography (IMAC) is widely used to purify proteins (Experiment 4). Find two proteins that have recently been purified by this technique and briefly describe the methods for isolation. 

El 10. What amino acid residues in proteins bind to chromatography gels containing immobilized metal ions ... 

R. Boyer,/. Chem. Educ. 68, 430-432 (1991). Purification of Milk Whey a-Lactal-bumin by Immobilized Metal-Ion Affinity Chromatography. ... 

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