Immobilized lipoxygenase

The ability of free or immobilized lipoxygenase to introduce oxygen derived from the air into polyunsaturated fatty acids in media containing organic solvent and aqueous buffer has been investigated [9,56]. The influence of many parameters was tested upon the degree of oxygenation in biphasic systems [36,108]. 

Yotova L, Marinkova D (2008) Application of immobilized lipoxygenase in the biosensors design for afiatoxin Bl. BioPS 08 November 4-5,111.37-III.46... 

Fig. 1. Comparison of the immobilized ( — ) and free ( — ) lipoxygenase at room temperature. At the times indicated, the samples were assayed to measure the lipoxygenase. Zero day of free enzyme or immobilized lipoxygenase is expressed as 100%...

Fig. 2. Reusability of the immobilized lipoxygenase. The immobilized beads were incubated with linoleic acid for five cycles. The activity of lipoxygenare during the first cycle is expressed as 100%.

Piazza, G.J., Brower, D.P. and Parra-Diaz, D. (1994) Synthesis of fatty acid hydroperoxide in the presence of organic solvent using immobilized lipoxygenase, Biotechnol Appl Biochem. 19, 243-252. 

Lipoxygenase (EC 1.13.11.12) catalyzes the dioxygenation of polyunsaturated fatty acids to form their corresponding hydroperoxides. Reduc derivatives of these have the potential to replace ricinoleic acid in industrial products. Currently ricinoleic acid is obtained from castor oil, a commodity that is imported into the U.S. at the level of thirty thousand metric tons per year (1). A method for immobilizing lipoxygenase was developed, and the use of this immobilized preparation to produce die hydroperoxide of linoleic acid (HPOD) in aqueous buffer-organic solvent mixtures was investiga. ... 

Further enzyme-catalyzed lipoxygenation reactions using immobilized lipoxygenase in organic solvents or converting even non-natural substrates were also reported. An overview over the synthetic potential of lipoxygenases for asymmetric oxidations is given in the comprehensive... 

Immobilized soybean lipoxygenase With carbonyldiimidazole-activated matrix termed Reacti-Gel(g) 13-Hydroperoxy-acid from linoleic acid Octane-borate buffer pH9 (5/2) No surfactant is required 24... 

The immobilization was performed with 1% solution of microbial lipoxygenase [E.C 1.13.11], from Pseudomonas sp., produced by Biovet, Peshtera, Bulgaria stirring it constantly in a closed container at t = 25°C for 21 h in phosphate buffers with pH = 5, 6 and 8 and acetate buffer pH = 4. After the completion of the procedure, the carrier granules were washed many times in buchner funnel with distilled water and 1 M NaCl until there was no absorption at /V = 280 mn. 

Fig. 5 Stability of microbial lipoxygenase immobilized on granulated polymer carrier in time...

Immobilized soybean lipoxygenase Immobilized soybean lipoxygenase... 

Battu, S., Rabinovitch-Chable, H. And Beneytout, J. L. (1994) Effectiveness of talc as adsorbent for purification and immobilization of plant lipoxygenases, J. Agric. Food Chem. Vol. 42, 2115-2112. 

IMMOBILIZATION OF LIPOXYGENASE IN AN ALGINATE-SILICATE SOLGEL MATRIX FORMATION OF FATTY ACID HYDROPEROXIDES... 

In our study, the reusability of LOX immobilized in calcium-alginate beads was determined by repeatedly using the same beads for five successive reactions with linoleic acid (LA) (Fig. 2). The LOX activity was measured after each cycle, and the beads were recovered and washed with sodium borate buffer before reuse. To initiate the next cycle of oxidation, LA was added to the incubation mixture containing the recovered beads. The data (Fig. 2) demonstrates that LOX immobilized in beads can be reused at least five times without substantial loss in enzyme activity. In contrast, free lipoxygenase is typically inactivated by hydroperoxide accumulation and the partial anaerobic condition that develops in the reaction mixture (8). 

In this study, lipoxygenase was successfully entrapped by a novel immobilization method within a calcium-alginate silicate gel matrix. The entrapped lipoxygenase had stability better than LOX immobilized by other methods. The immobilized preparation also was reusable. This procedure should enhance the potential of LOX as a biocatalysts in the preparation of oxygenated fatty acid derivatives that have unique chemical and physical properties. 

Immobilization of Lipoxygenase in Alginate-Silicate Sol-Gel Matrix Formation of Fatty Acid Hydroperoxides. 

Parra-Diaz D, Brower DP, Medina MB, and Piazza GJ. A method for immobilization of lipoxygenase. Biotech and Applied Biochem 1993 18 359-67. 

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