Partitioning into Immobilized Liposomes

Compounds that show low but intrinsic absorption can be optimized by various galenic techniques and procedures. However, those which possess no absorption ability at all cannot be optimized by such procedures. New strategies have been developed for novel drag delivery systems to control drag release, transport, and absorption across mucosal membranes. A special class of modifiers are amphiphilic 

---

C Lagerquist, F Beigi, A Karlen, H Lennernas, P Lundahl. Effects of cholesterol and model transmembrane proteins on drug partitioning into lipid bilayers as analyzed by immobilized-liposome chromatography. J Pharm Pharmacol 53 1477-1487, 2001. 

F Beigi, Q Yang, P Lundahl. Immobilized-liposome chromatographic analysis of drug partitioning into lipid bilayers. J Chromatogr A 704 315-321, 1995. 

Immobilized artificial membrane (lAM) stationary phase consists of a monolayer of phospholipid covalently immobilized on an inert silica support. The lAM stationary phase mimics the lipid environment found in cell membranes, and it can be used for elucidating drug-membrane interactions. The interaction of catechins, flavones, flavonols, anthocyanidins, and anthocyanins with phosphatidylcholine was investigated by HPLC with an lAM colunm. The lAM partition coefficients of the flavonoids correlated well with the amounts flavonoids incorporation into the liposomes [42]. 

---