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Iminohydrolase

Lathyrus sativus (enzyme has inherent activities of agmatine iminohydrolase, putrescine transcarbamylase, ornithine transcarbamylase and carbamate kinase [1]) [1]... [Pg.275]

El 15 Grisley, D., Mauck, L. (1983). An iminohydrolase procedure for the determination of creatinine in serum and urine. Clin. Chem. 29, 1178, Abstr. 114. [Pg.277]

For the diagnosis of kidney function, creatinine determinations in serum are increasing in popularity over urea measurements, since the creatinine level is not affected by a high protein diet or by metabolic rate, as is the case for urea. Enzymatic methods use either creatinine amido-hydrolase (EC 3.5.2.10) or creatinine iminohydrolase (EC 3.5.4.21) ... [Pg.174]

Danielsson (1982) proposed a reactor with immobilized creatinine iminohydrolase in a thermistor unit. Although the measuring range was 0.01—10 mmolA creatinine, the sensitivity was still too low for the assay... [Pg.174]

The very same enzymes have been combined by Mascini et al. (1985a) in an FIA system. Creatinine iminohydrolase was immobilized on the inner wall of nylon tubing (diameter 1 mm, length 1 m) and the ammonia liberated in the enzymatic reaction was measured with an NH3 electrode. Owing to the low sensitivity of the indicator electrode, the linear range was only 0.01-0.2 mmolA. [Pg.175]

Meyerhoff and Rechnitz (1976) developed a potentiometric creatinine sensor by inclusion of creatinine iminohydrolase between the gas-permeable membrane of an ammonia electrode and a dialysis membrane. Since the specific activity of the enzyme used was very low, 0.1 U/mg, only 43 mU could be entrapped at the electrode. Therefore the sensor was kinetically controlled and reacted to addition of the enzyme activator tripolyphosphate by an increase in sensitivity from 44 mV to 49 mV per concentration decade and a corresponding decrease of the detection limit. These effects agree with theoretical considerations of reaction-transport coupling. The samples were treated with a cation exchanger to remove endogenous serum ammonia. [Pg.175]

Figure 22 Biosynthesis of polyamines through the arginine decarboxylase pathway. Putrescine derived from L-arginine through the action of three successive enzymes arginine decarboxylase (ADC), agmatine iminohydrolase (AIH), and N-carbamoylputrescine amidohydrolase (NCPAH). Figure 22 Biosynthesis of polyamines through the arginine decarboxylase pathway. Putrescine derived from L-arginine through the action of three successive enzymes arginine decarboxylase (ADC), agmatine iminohydrolase (AIH), and N-carbamoylputrescine amidohydrolase (NCPAH).
Plasma and urinary creatinine are commonly measured by the colorimetric alkaline picrate method of Jaffe or by alternative enzymatic methods. Enzymatic methods use creatinine amidohydrolase or creatinine iminohydrolase and are more specific for creatinine. The measurement of plasma creatinine may be affected by endogenous noncreatinine chromogens (e.g., bilirubin and ketones) this can overestimate plasma creatinine in dogs by up to 45% and to an even greater extent in rats... [Pg.75]

Reaction 5 is catalyzed by arginine decarboxylase which has been found in barley (Smith, 1963). Agmatine can be converted to A-carbamoyl putrescine (reaction 6) by agmatine iminohydrolase (E.C. 3.5.3.12). This enzyme has been found in extracts of maize leaves and sunflower (Smith, 1969). The further hydrolysis of A/ -carbamyl putrescine to putrescine (reaction 7) is catalyzed by A-carbamyl putrescine amidohydrolase which has been found in barley leaf extracts (Smith, 1965). The enzyme activities catalyzing reactions 5 and 7 are increased several-fold in K-deficient barley leaves (Smith, 1963, 1965) which correlates well with the high level of putrescine accumulation in K-deficient barley. Further metabolism of putrescine is discussed by Smith, this series, Vol. 7, Chapter 9. [Pg.556]

Putrescine accumulation has been investigated in some detail and studies with radioactive precursors (see e.g.. Smith and Richards, 1%2 Smith, 1965) have established that it is synthesized from arginine (see Fig. 1). The mechanism underlying putrescine accumulation is uncertain although two of the biosynthetic enzymes, arginine decarboxylase, and iV-carbamyl pqtres-cine amidohydrolase exhibit two- to fourfold increases under conditions of K deficiency (Smith, 1%5). No increase was found to occur in the activity of agmatine iminohydrolase (Smith, 1969). [Pg.611]

The second most common method used in automated analyzers is based on the use of enzymes to produce a compound that will react with a substance provided to form a colored product. For example, creatinine iminohydrolase (creatinine deiminase) catalyzes the conversion of creatinine to N-methyT hydantoin and ammonia. This reaction can be carried out in a flow injection analysis system setup so that the ammonia diffuses into an acceptor stream containing a pH indicator, the color changes of which are measured by diffuse reflectance. [Pg.741]

To create a potentiometric creatinine sensor, creatinine iminohydrolase, which catalyzes the production of ammonia from creatinine, can be immobilized on the surface of an ammonium ion selective electrode. There is no interference from creatine but some from the ammonium ions in blood and urine. [Pg.742]

Another biosensor is based on an ion-sensitive field-effect transmitter. Creatinine iminohydrolase is immobilized on the gate by cross-linking with bovine serum albumin in glutaraldehyde vapor. Creatinine iminohydrolase catalyzes the formation of N-methyl-hydantoin (and ammonia) from creatinine. The differential signal between a reference and sensing chip is proportional to creatinine concentration. [Pg.742]

Creatinine iminohydrolase / /-Methylhydantoin amidohydrolase Pyruvate kinase Lactate dehydrogenase NAD +... [Pg.742]

Creatinine iminohydrolase Creatinine iminohydrolase /V-Methylhydantoin amidohydrolase W-Carbamoylsarcosine amidohydrolase Sarcosine oxidase Red product of peroxidase on 4-aminoantipyrine and phenol Ammonia... [Pg.742]


See other pages where Iminohydrolase is mentioned: [Pg.66]    [Pg.33]    [Pg.205]    [Pg.271]    [Pg.272]    [Pg.275]    [Pg.279]    [Pg.50]    [Pg.1388]    [Pg.170]    [Pg.171]    [Pg.176]    [Pg.381]    [Pg.382]    [Pg.145]    [Pg.4]    [Pg.41]    [Pg.175]    [Pg.216]    [Pg.294]    [Pg.496]    [Pg.125]    [Pg.143]    [Pg.143]    [Pg.282]    [Pg.85]    [Pg.259]    [Pg.378]    [Pg.612]    [Pg.178]    [Pg.180]    [Pg.249]   
See also in sourсe #XX -- [ Pg.269 ]




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Agmatine iminohydrolase

Agmatine iminohydrolase, putrescine

Creatinine iminohydrolase

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