Sarcosine oxidase

In this section, we summarize information about five Ci enzymes involved in metabolism of 5-formyl-THF (5-CHO-THF) and formate, whose existence was predicted from genomics data. 5-Formyltetrahydrofolate cycloligase (5-FCL) and S-formylglutathione hydrolase have since been shown to catalyze the anticipated reactions the other three enzymes (10-formyl-THF deformylase, formamidase, and sarcosine oxidase) are still putative. 

REUBER, B.E, KARL, C., REIMANN, S.A., MIHALDC, S.J., DODT, G. Cloning and functional expression of a mammalian gene for a peroxisomal sarcosine oxidase, J. Biol. Chem., 1997, 272, 6766-6776. 

KOYAMA, Y., OHMORI, H. Nucleotide sequence of the Escherichia coli solA gene encoding a sarcosine oxidase-like protein and characterization of its product, Gene, 1996, 181, 179-183. 

CHIKAYAMA, M., OHSUMI, M., YOKOTA, S., Enzyme cytochemical localization of sarcosine oxidase activity in the liver and kidney of several mammals, Histochem. Cell Biol., 2000,113,489-495. 

Horse liver alcohol dehydrogenase, F93W mutant with 1224 also mutated to G,A,V,L. hydride transfer from benzyl alcohol to NAD Heterotetrameric sarcosine oxidase of Arthrobacter sp. 1-IN, proton transfer from adduct of FAD with sarcosine-(CH3) and sarcosine-(CD3)... 

Harris, R.J., Meskys, R., Sutcliffe, M.J. and Scmtton, N.S. (2000). Kinetic studies of the mechanism of carbon-hydrogen bond breakage by the eterotetrameric sarcosine oxidase of Arthrobacter sp. 1-IN. Biochemistry 39, 1189-1198... 

SALICYLATE 1-MONOOXYGENASE SARCOSINE OXIDASE STEROID HYDROXYLASES SULFITE OXIDASE SULEUR DIOXYGENASE THYMIDINE 2 -HYDROXYLASE TOLUENE DIOXYGENASE XANTHINE OXIDASE... 

SARCOSINE DEHYDROGENASE SARCOSINE OXIDASE SATURATION Saturation function,... 

In an early application, an enzyme electrode system was reported for the determination of creatinine and creatine, using a combination of creatinine amidohy-drolase, creatine amidinohydrolase and sarcosine oxidase, co-immobilized on an asymmetric cellulose acetate membrane. Thus, the hydrogen peroxide produced was detected to give a quantitative measure of creatine and creatinine in biological fluids [70]. 

Different possibilities for conducting enzymatic assays on microchip platforms including pre-, on- or post-column reactions have been reviewed [156]. An enzymatic assay (employing creatininase, creatinase and sarcosine oxidase) has also been developed in a microchip for analysing renal marks such as creatinine and creatine as well as p-aminohippuric acid and uric acid [157]. 

Willie, A., Edmondson, D. E., and Joms, M. S., 1996, Sarcosine oxidase contains a novel covalently bound FMN, Biochemistry 35 529295299. 

An interesting application for the oxidation of organic compounds is of electrochemical nature. Octacyano complexes have been used to monitor redox enzymes such as lactate oxidase (from Pediococcus sp.) and sarcosine oxidase (from Arthrobactersp.) in a suitable electrochemical system (114). Two equivalents of [M(CN)g] can, for example, be oxidized at the electrode surface to [M(CN)g], which in turn can oxidize the flavoproteien to its oxidized form. This in turn reacts with, for example, L-lactic acid to produce pyruvic acid. 

E480 Roberts, R.T., Alexander, N.M. and Kelner, M.J. (1988). Definitive liquid-chromatographic demonstration that A-ethylglycine is the metabolite of lidocaine that interferes in the Kodak sarcosine oxidase-coupled method for creatinine. Clin. Chem. 34, 2569-2572. 

Creatine and creatinine Creatininase, creatinase, and sarcosine oxidase 30 mM... 

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