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The two rearrangement products from the given substituted methylenecyclobutane are seen to be related as mirror images, which makes interpretation of the rearrangement of such an optically active system subject to much uncertainty a mixture of antipodes in the product might imply either a lack of stereochemical specificity or a competitive operation of both fully stereoselective rearrangement pathways. [Pg.7]

Figure 4.9. Staining of Proteins After Electrophoresis. Proteins subjected to electrophoresis on an SDS-polyacrylamide gel can be visualized by staining with Coomassie blue. [Courtesy of Kodak Scientific Imaging Systems.]... Figure 4.9. Staining of Proteins After Electrophoresis. Proteins subjected to electrophoresis on an SDS-polyacrylamide gel can be visualized by staining with Coomassie blue. [Courtesy of Kodak Scientific Imaging Systems.]...
Fig. 21 STM simulations for systems subject to a static vs. dynamic JT effect. The top row corresponds to the excited state and the bottom to the ground state. In (a), infinitely strong coupling locks the molecule into one particular well. Finite but strong coupling (so that the system jumps between three wells) is shown in (b). Further reduction in localisation leads to essentially free pseudorotation, producing the time-averaged images in (c)... Fig. 21 STM simulations for systems subject to a static vs. dynamic JT effect. The top row corresponds to the excited state and the bottom to the ground state. In (a), infinitely strong coupling locks the molecule into one particular well. Finite but strong coupling (so that the system jumps between three wells) is shown in (b). Further reduction in localisation leads to essentially free pseudorotation, producing the time-averaged images in (c)...
In film-based imaging, spatial resolution is often assessed by determining the limiting resolution in terms of line-pairs/mm from a bar pattern. This is a subjective test, however, and is not very useful in the analysis of complex imaging systems. [Pg.10]

Lastly, the pricing of remanufactured products has become a subject of debate. Traditionally, remanufactured products have been priced at a lower value than new products due to the reused components. For instance, Siemens remanufactured imaging systems price runs about two-thirds that of a new unit, which are usually priced between 160,000 and 170,000. However, it is still in discussion whether remanufactured products should receive a lower valuation. A diversity of variables such as certification of the final product, number of parts replaced or upgraded, and demand for remanufactured components must be taken into account in this decision. [Pg.1046]

The phantom was subjected to illumination from the laser source and the speckle pattern from the target is imaged by the imaging system explained in section B. [Pg.444]

Figure 5, RT-PCR analysis of iNOS mRNA. (A) Cells were treated with different concentrations of camosol and LPS (I ptg/mL) for 12 h. 5 fig of total RNA was subjected to reverse transcription-polymerase chain reaction (RT-PCR) and PCR product was resolved in 1% agarose gel C, control (B) Data was quantified using a densitometer (IS-100 Digital Imaging System), and the relative level observed with LPS alone is set at 1. Figure 5, RT-PCR analysis of iNOS mRNA. (A) Cells were treated with different concentrations of camosol and LPS (I ptg/mL) for 12 h. 5 fig of total RNA was subjected to reverse transcription-polymerase chain reaction (RT-PCR) and PCR product was resolved in 1% agarose gel C, control (B) Data was quantified using a densitometer (IS-100 Digital Imaging System), and the relative level observed with LPS alone is set at 1.
FIGURE 11 Coronal head image. The subject was in the same position as in Fig. 10 but by interchanging the gradients used for seleotive excitation an image of a slice at right angles to that shown in Fig. 10. (Courtesy of GE Medical Systems.)... [Pg.252]

Fig. 2 Increasing content recovery by coupling luciferase-based assays with high-throughput biochemical readouts. The same cell line subjected to the luciferase-based assay protocol (HCT116 cells) is evaluated here for its reliability In reporting a biochemical readout (p53 expression) by dot blot analysis. Cells transfected with indicated expression construct and pathway reporters in a 96-well culture plates were lysed 48 h posttransfection. Following luciferase activity measurements (data not shown), protein from spent lysates was immobilized on nitrocellulose using a liquid handler and filtration manifold, (a) p53 and p-actin protein levels detected using protein-specific antibodies, infrared fluorescent dye-coupled secondary antibodies (with emissions at 680 and 800 nM), and the Li-COR imaging system. Columns of lysate corresponding to cells transfected with p53 DNA are boxed, (b) Quantification of the p53 to p-actin protein ratio... Fig. 2 Increasing content recovery by coupling luciferase-based assays with high-throughput biochemical readouts. The same cell line subjected to the luciferase-based assay protocol (HCT116 cells) is evaluated here for its reliability In reporting a biochemical readout (p53 expression) by dot blot analysis. Cells transfected with indicated expression construct and pathway reporters in a 96-well culture plates were lysed 48 h posttransfection. Following luciferase activity measurements (data not shown), protein from spent lysates was immobilized on nitrocellulose using a liquid handler and filtration manifold, (a) p53 and p-actin protein levels detected using protein-specific antibodies, infrared fluorescent dye-coupled secondary antibodies (with emissions at 680 and 800 nM), and the Li-COR imaging system. Columns of lysate corresponding to cells transfected with p53 DNA are boxed, (b) Quantification of the p53 to p-actin protein ratio...

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See also in sourсe #XX -- [ Pg.1053 ]

See also in sourсe #XX -- [ Pg.1053 ]




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