IMAGING OF SMALL MOLECULES

In the literature these studies are classified as imaging mass spectrometry (IMS) and defined as the investigation of the chemical profile of a sample surface with a submicron lateral resolution and chemical specificity. The main aim is to use the power of mass spectrometry techniques to create chemical images showing the distribution of compounds ranging in size from atomic ions and small molecules to large proteins. 

The ideal imaging method should enable simultaneous detection and identification of known and unknown compounds present in the studied sample. During analysis, analyte atoms and molecules are removed from the sample surface, in most cases only 

Mass Spectrometry. Edited by Ekman, Silberring, Westman-Brinkmalm, and Kraj Copyright 2009 John Wiley Sons, Inc. 

Mainly three ionization methods are applied in mass spectrometry  

In this chapter a number of the recent applications of imaging of small molecules with the use of SIMS imaging will be presented. 

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Staff New Projectors, Hughesnews, 1, Culver City, California (February 21, 1992). Staff Imaging Technologies, Inscribing Science, Camera Obscura, 28 (1992). Vager, Z., R. Naaman, and E.P. Kauter Coulomb Explosion Imaging of Small Molecules. Science, 426 (April 28, 1989). 

Garrett T, Prieto-Conaway M, Kovtoun V, Bui H, Izgarian N, Stafford G, Yost R (2007) Imaging of small molecules in tissue sections with a new intermediate-pressure MALDI linear ion trap mass spectrometer. Int J Mass Spectrom 260 166-176. doi 10.1016/j. ijms.2006.09.019... 

Liu Q, Guo Z, He L (2007) Mass spectrometry imaging of small molecules using desorption/ ionization on silicon. Anal Chem 79 3535-3541. doi 10.1021/ac0611465... 

Reyzer, M.L. and Caprioli, R.M., MALDI-MS-based imaging of small molecules and proteins in tissues, Curr. Opin. Chem. Biol., 11, 29, 2007. 

These studies have demonstrated that PET imaging of small molecules radiolabeled with positron emitters can add value to classical studies of new drugs. The PET radiolaheling studies provide very important tissue pharmacokinetics information. It is worth noting, however, that the achievable pharmacokinetic parameters may be limited for three reasons ... 

Ridenour, W.B. Snel, M.F. Claude, E. Mclean, J.A. Frappier, S.L. Caprioli, R.M. Simultaneous imaging of small molecules from thin tissue sections using MALDI travelling wave ion mobility-mass spectrometry. Proc. 56th ASMS Conference on Mass Spectrometry and Allied Topics, Denver, CO, June 1-5, 2008, MP 139. 

Mass Spectrometry Imaging of Small Molecules Using Matrix-Enhanced Surface-Assisted Laser Desorptlon/lonizatlon Mass Spectrometry (ME-SALDI-MS)... 

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