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Image resolution superresolution

In the quest for optimal FRET resolution in three dimensions [48, 49], one requires sophisticated algorithms for analyzing complex distributions [48, 50, 51], An issue of particular relevance when multiple probes [52-54] and multiparametric detection [26, 55] are employed. Strategies for spatial superresolution [56] have spawned a family of acronyms RESOLFT, STED, STORM, astigmatic STORM, PALM, fPALM, sptPALM, PALMIRA ([57-59] and other references), some of which involve systematic photoconversion or destruction. Undoubtedly, these techniques will be applied systematically to FRET imaging, and, conversely, one can anticipate that FRET mechanisms will be exploited for achieving superresolution. [Pg.495]

However, finding out the position of an object with arbitrary precision is not the same as resolution, which is about separating similar objects at small distances. Localization per se cannot provide superresolution. This is also why, although it had been known and used for decades [109,110] and even routinely applied to single molecules [125,126], localization alone has not provided nanoscale images. (Note that in spite the use of localization in the 1980 s and earlier, near-field optical microscopy still seemed to be the only way to attain nanoscale resolution up to the early 1990 s.) Resolution clearly requires a criterion to discern objects or molecules, the simplest of which is bright vs. dark. ... [Pg.389]

The use of a single LED source has its advantages that the microscope developed is simple, small and portable. But it also suffers from some disadvantages like poor axial resolution because of under-sampling. To overcome this pixel superresolution (SR) techniques have been followed, where a super-resolution picture is obtained from a series of low-resolution images. Each image in... [Pg.2567]

Although image acquisition systems improve their spatial resolution, there are instrumental limits related to this parameter. Superresolution strategies are mathematical procedures devoted to obtain spatial descriptions of the sample surface that go beyond the limits of the instrumental spatial resolution [135,136]. [Pg.104]

Each of these images was shifted from the closest one by a motion step of 0.6 pm in the x- ory-direction. Shifts in the x- andy-direction with respect to the original image went from 1 to 6 times 0.6 pm. After resolution of the multiset formed by the 36 images, three contributions were modeled with clear spectral signatures. By application of the superresolution postprocessing to the three sets of low spatial... [Pg.105]

Different fluorescence microscopy techniques are commonly used to explore the organization of the microtubule cytoskeleton. The resolution of these techniques is, however, limited by diffraction to approximately 250 nm, which makes them not suitable for nanoscale mapping of microtubule properties. Superresolution microscopy techniques that rely on single molecule localization (Single Molecule Localization Microscopy SMLM) combine high protein specificity, multi-color imaging, and a resolution in the order of 5-50 nm, making it an ideal tool to study the neuronal cytoskeleton and its properties. [Pg.389]


See other pages where Image resolution superresolution is mentioned: [Pg.548]    [Pg.48]    [Pg.49]    [Pg.49]    [Pg.51]    [Pg.371]    [Pg.373]    [Pg.388]    [Pg.35]    [Pg.126]    [Pg.104]    [Pg.104]    [Pg.105]   
See also in sourсe #XX -- [ Pg.104 , Pg.105 ]




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