Image analysis immunohistochemistry

Note that although immunohistochemistry is contributing significantly to clinical information, in the absence of quantitation, this method is subjective and prone to intra- and interobserver variations. To assure the reproducibility of histopathological results and then-correct evaluation, at least an assessment of the percentage of positive cells and both the staining pattern and intensity must be made. If available, computer-assisted image analysis... 

Hernandez-Blazquez, F. J., Habib, M., Dumollard, J.-M., Barthelemy, C., Benchaib, M., de Capoa, A., and Niveleau, A. 2000. Evaluation of global DNA hypomethylation in human colon cancer tissues by immunohistochemistry and image analysis. Gwi47 689-693. 

Key Words Immunohistochemistry digital image analysis morphological parameters cell area optical density cytoskeletal proteins. 

Taatjes, D. J. Wadsworth, M. P Schneider, D. J. Sobel, B. E. Improved quantitative characterization of atherosclerotic plaque composition with immunohistochemistry, confocal fluorescence microscopy, and computer-assisted image analysis. Histochem. Cell Biol. 2000,113,161-173. 

Fig. 10 Confocal microscopy double IMF of neuroblastoma B50 cells. Staining for mitochondria green) and lysosomes (red) in (a) controls, (b) after 48-h treatment with cisPt, and (c) recovery. Images show that, after cisPt, lysosomes and mitochondria are distributed in cytoplasmic clusters compared to control, but do not show colocalization. During recovery, cells assume a morphology similar to control cells and show an increase of lysosomes. Immunohistochemistry for LC3B (green) and lysosomes (red) in (d) control and (e) recovery cells. Graph analysis shows the fluorescence peak and colocalization of the two labels. DNA is counterstained with Hoechst 33258 (blue). Scale bars 20 pm...

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