Illuminants optical sorting

In one application, an epi-illuminated optical trap was used in conjunction with an inverted fluorescence microscope so that individual cells could be identified with FITC-conjugated antibodies and subsequently trapped for further micromanipulation (24). In the first part of the experiment, as a demonstration of a sorting technique, the trapped cells were transferred into a capillary tube, that was located in the Petri dish along with the cells. The optical trap was used to lift... 

An optical interferometric technique is used to measure the separation D, simultaneously providing also the liquid refractive index n and the curvature R. The mica sheets are made partially reflecting by evaporating a 40 -f 50 nm silver layer on the inner sides of the mica sheets. The cell is then illuminated by a collimated white light beam, in a direction perpendicular to both axis of the cylinders. The two partially reflective silver layers form a sort of three-layer curved interferometer selective transmission occurs only for the spectral components resonating with the mica/liquid/mica cavity . The transmitted wavelengths A are analyzed by an interferometer with a resolution better than 0.1 nm, to get information about the optical properties of the layers, especially the thickness D and the index n of the liquid. 

Optofluidic Manipulation of Biological Molecules, Fig. 4 (a) Cells within the microchannel can be localized and classified with bright-field (green) and/or fluorescence (blue) illumination through a high-NA objective. Once a cell is classified, it is moved by optical tweezers (red) to a streamline of laminar flow which leads to the outlet for sorted cells, (b, c) Photographs of microfluidic chamber inserted into its chip holder and connected to... 

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