Ibuprofen gradient separation

Figure 3.10 Gradient separation of ibuprofen metabolites from a human urine sample on a 2.1 by 100mm reversed-phase C18 column using a TriVersa Nano-Mate coupled with a Waters Q-TOF micro mass spectrometer. (A) Extracted ion chromatogram for carboxy-ibuprofen-diglucuronide with MH+ at m/z 589.3 and (B) base peak intensity chromatogram. LC fractions were collected simultaneously every 15 s into a 384-well plate.

Fig. 5. Gradient separation of lidocaine, prednisolone, naproxen, amitriptyline, and ibuprofen. Column 2.1 mm x 30 mm XTerra MS 3.5 pm.

Ahrer et al. [69] developed methods for the determination of drug residues in water based on the combination of liquid chromatography (LC) or capillary electrophoresis (CE) with mass spectrometry (MS). A 2 mM ammonium acetate at pH 5.5 and a methanol gradient was used for the HPLC-MS allowing the separation of a number of drugs such as paracetamol, clofibric acid, penicillin V, naproxen, bezafibrate, carba-mazepin, diclofenac, ibuprofen, and mefenamic acid. Apart from the analytical separation technique, water samples have to be pretreated in order to get rid of the matrix components and to enrich the analytes the usual way to accomplish this aim is to perform a solid-phase extraction... 

Various reversed phase HPLC systems were studied for the separation of ibuprofen, pseudoephedrine HCl, chlorpheniramine maleate and nipagen at various flow rates under both isocratic and gradient conditions. The best separation was accomphshed using three steps gradient elution with acetoninile/pH = 3 phosphate buffer. Figures... 

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