HypB protein

Multiple alignment of HypB proteins from Eubacteria and Archaea... 

Olson, J. W., Fu, C. and Maier, R. J. (1997) The HypB protein from Bradyrhizobium japon-icum can store nickel and is required for the nickel-dependent transcriptional regulation of hydrogenase. Mol. Microbiol., 24, 119-28. 

Figure 6.3. Alignments of regions identified to be important in nickel accessory proteins for maturation of the final nickel sink, the nickel-containing enzyme. A, The His-rich regions of known or putative HypB proteins and the UreE and CooJ proteins from various organisms. Reprinted with permission from Olson and Maier (2000). B, Alignment of the G motifs from known or putative HypB proteins and the related nucleotide-binding P-loop residues (in the G1 region) of CooC and UreG. (Adapted from Olson and Maier (2000).

Table 6.2. Characteristics of full-length and mutated hypB proteins.

Fu C, Olson JW, Maier RJ. 1995. HypB protein of Bradyrhizobium japonicum is a metal-binding GTPase capable of binding 18 divalent nickel ions per dimer. Proc Natl Acad Sci USA 92 2333-7. 

HypB protein, 47 289 HypC protein, 47 289 Hyperfine coupling, 13 149-178 anisotropic, 13 150-161 Hyperfine coupling anisotropic dipolar, 13 150-154 nuclear Zeeman interaction, 13 155 quadrupole interaction, 13 154, 155 factors affecting magnitude of metal influence of charge on metal, 13 169-170 isotropic and anisotropic, 13 166-170 libration, 13 170... 

Maier, T., Jacobi, A., Sauter, M. and Bock, A. (1993) The product of the hypB gene, which is required for nickel incorporation into hydrogenases, is a novel guanine nucleotide-binding protein./. Bacterial., 175, 630-5. 

Rey, L., Imperial, J., Palacios, J.-M. and Ruiz-Argueso, T. (1994) Purification of Rhizobium leguminosarum HypB, a nickel-binding protein required for hydrogenase synthesis. J. Bacterial., 176, 6066-73. 

Olson JW, Mehta NS, Maier RJ. 2001. Requirement of nickel metabohsm proteins HypA and HypB for full activity of both hydrogenase and urease in Helicobacter pylori. Mol Microbiol 39 176-182. 

Fig. 6. Schematic representation of the biosynthetic pathway associated with the assembly of the NiFe-hydrogenase catalytic metal center. The precursor to the large subunit is represented by pre-HycE-Fe, and the CO (and/or CN) ligands are provided by the multi-protein complex formed between HypE, HypC and HypD. The source of the ligands to HypE is carbamoyl phosphate, and the insertion reaction is catalyzed by HypF. The Ni atom is inserted into the pre-HycE-Fe-CO-CN-HypC complex in a reaction catalyzed by HypA and HypB. Finally, the Hycl endopeptidase processes the C-terminus of the pre-HycE-Fe-CO-CN-HypC complex, yielding the mature NiFe-hydrogenase large subunit.

Rey L, Imperial J, Palacios JM and Ruizaegueso T (1994) Purification of Rhizobiumleguminosarum HypB, a nickd-binding protein required for hydro-genase synthesis. J Bacteriol 176 6066-6073. 

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