8-Hydroxy-1,3,6-pyrenetrisulfonate

CAS 6358-69-6 EINECS/ELINCS 228-783-6 Synonyms 8-Hydroxy-1,3,6-pyrenetrisulfonic acid trisodium salt Solvent green 7 Trisodium... 

HPTS assay A technique that reports with 8-hydroxy-1,3,6-pyrenetrisulfonate (pyranine) on pH changes within vesicles. 

Carbon dioxide dissolved in sea water Fiber dipped in HPTS solution Aqueous solution of 8-hydroxy-1,3,6-pyrenetrisulfonic acid-trisodium salt Fluorescence 450 530 — O-fiOO ppm 5 min 58... 

Pyranin and Prodan. Water consumption was probed during the early stage of the sol-gel reaction of TMOS by using excited state proton transfer of PYRANIN (8-hydroxy-1,3,6-pyrenetrisulfonic acid trisodium salt Fig. 20-8) (Kaufman, 1988). This PYRANIN was applied to study aminosilicate matrix (Pouxviel, 1989) and as an in situ fluorescence... 

In order to follow the reaction in real-time, we devised an experimental system in which the enzyme is encapsulated, together with a fluorescence probe, into giant liposomes (diameter larger than 10 xm) in this way, the reaction dynamics over time can be easily monitored by an optical microscope equipped with a fluorescence module. Pyranine (8-hydroxy -1,3,6-pyrenetrisulfonate), has been chosen as the fluorescent probe, since its emission intensity (at 510 nm) is strongly dependent upon the pH of the solution, over the range 6-10. Pyranine... 

Barnadas-Rodriguez, R. Effect and mechanism of association of 8-Hydroxy-1,3,6-pyrenetrisulfonic acid to chitosan Physicochemical properties of the complex. Macwmol. Chem. Phys. 2013, 214, 99-106. 

Hydroxypropyl)phosphonothioic acid 0,0-dibulyl ester, see D-00234 8-Hydroxy-1,3,6-pyrenetrisulfonic acid, H-00517... 

Other fluorescent colorants can be used to produce pH, pNHs, and pCX)2 optical sensors. One example is the trisodium salt of 8-hydroxy 1,3,6-pyrenetrisulfonic acid (called HOPS A or HPTS) whose basic form can be excited at 470 nm to emit fluorescence at 510 nm [203, 204]. The CX)2 optical sensor requires a silicone membrane to contain a bicarbonate solution, just like potentiometric pC02 electrodes. A further similarity with potentiometric electrodes is that the pH, pNHs and pC02 optical sensors serve as the bases for enzyme sensors. The appropriate enzymatic membrane is simply attached. The optical sensor for penicillin uses penicillinase to catalyse the transformation of penicillin into penicilloic acid. The acid is detectable with a pH optical sensor that uses fluorescein isothiocyanate (FITC) as a colored indicator [205]. 

A limited number of commercially available hydroxyarenes, including hydroxy-pyrenetrisulfonate ( pyranine , HPTS), have somewhat higher photoacidities than naphthols and have been used as photoacids for experiments requiring instant changes in acidity, e.g., pH jump. Again, HPTS is limited to aqueous solvents. 

Trisodium 8-hydroxy-l,3,6-pyrenetrisulfonate [6358-69-6J M 488.8, m >300(dec). Purified by chromatography with an alumina column, and eluted with -propanol-water (3 1, v/v). Recrystd from aqueous acetone (5 95, v/v) using decolorising charcoal. 

Ratiometric luminescent probes make a smart use of the excitation wavelength effect on the emission intensity for extended optosensor performance. For example, the fluorescence from 8-hydroxy-l,3,5-pyrenetrisulfonate (HPTS) and other pH-sensitive dyes in water comes only from its (photo)excited basic form, but the absorption spectra of HPTS and PTS (pAa 7.3) differ considerably (Figure 3). 

Figure 3. Absorption/fluorescence features of 8-hydroxy-l,3,5-pyrenetrisulfonate (HPTS) in water at different pH values (dark solid line absorption at pH 2 light solid line absorption at pH 11 dashed line emission at pH 11 dotted line emission at pH 2).

Hydroxy-l,3,6-pyrenetrisulfonic add, trisodium salt [6358-59-6] Cl 59040 Xmax 403 nm, UR2700000 ... 

SWCNTs were modified with several water-soluble pyrene derivatives (1-pyreneacetic acid, 1-pyrenecarboxylic acid, 1-pyrenebutyric acid, and 8-hydroxy-l,3,6-pyrenetrisulfonic acid) bearing negatively charged ionic head-groups, to act as anchors to several pyridium headgroups present in a series of water-soluble metalloporphyrins [MP] + (M = Zn, Co, and Fe) [60a]. Interactions between the metalloporphyrins and SWCNTs proceeded through a combination of associative van der Waals (spacer-CNT) and electrostatic interactions (metalloporphyrins-spacer-CNT), as demonstrated by the combination of spectroscopic and surface techniques. 

P-Pyrene. See Pyrene Pyrene, 1-nitro-. See 1-Nitropyrene 1,3,6-Pyrenetrisulfonic acid, 8-hydroxy-, trisodium salt. See Solvent green 7 Pyretherm. See Resmethrin Pyrethrin. See Pyrethrin II Pyrethrum Pyrethrin I... 

Pyranine 1,3,6-Pyrenetrisulfonic acid, 8-hydroxy-, trisodium salt Trisodium 8-hydroxypyrene-1,3,6-trisulfonate... 

Similar classical countercations for the detection of anion antiport in U-tubes do not exist. However, most standard probes including lucigenin (see below) will work for this purpose (unpublished results). Many alternative methods for the detection of U-tube activity are conceivable (ion-selective electrodes, probes, etc). For the transport of charged molecules, standard probes such as 8-hydroxy-l,3,6-pyrenetrisulfonate (HPTS) and 5(6)-carboxyfluorescein (CF) have been used directly to detect anion transport, and p-xylene bis(pyridinium) bromide (DPX), or safranin O has been used to detect cation transport (see below for structures). ... 

Clement, N.R., Gould, J.M. Pyranine (8-hydroxy-l,3,6-pyrenetrisulfonate) as a probe of internal aqueous hydrogen ion concentration in phospholipid vesicles. Biochemistry 20(6), 1534-1538 (1981)... 

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