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Hydrophilic interaction HPLC

An interesting approach was proposed in Lobinski s group for the analysis of non-covalent Ni species in biological samples.49 The Ni species in aqueous plant tissue extracts were quantitative determined by SEC-ICP-MS in combination with ESI-ToFMS/MS after purification of Ni species by hydrophilic interaction HPLC (HILIC).49... [Pg.326]

Because of the development of analytical methods for determination of MC, especially in last decade, the most widespread analytical techniques for their determination are commercialized enzyme-linked immunosorbent assays and HPLC with diode array detectors of anion-exchange columns for this purpose were only sporadically reported with UV detection and isocratic or gradient ° elution. For anion-exchange stationary phase functionalized with diethylaminoethylene groups, better resolution for LR and YR was reported than for Cl8 columns.For separation of cyanobacterial toxins, including MC-LR and -RR, hydrophilic interaction HPLC with gel Amide-80 column and amide C16 column providing comparable resolution to that of conventional reversed-phase Cl8 columns were applied. [Pg.1479]

A new topic is now included Chapter 20 about quahty assurance. Part of it could be found before in chapter 19 but now the subject is presented much broadly and independent of Analytical HPLC . Two chapters in the appendix were updated and expanded by Bruno E. Lendi, namely the ones about the instrument test (now chapter 25) and troubleshooting (now chapter 26). Some new sections were created 1.7, comparison of HPLC with capillary electrophoresis 2.11, how to obtain peak capacity 8.7, van Deemter curves and other coherences 11.3, hydrophilic interaction chromatography 17.2, method transfer 18.4, comprehensive two-dimensional HPLC 23.3, fast separations at 1000 bar 23.5, HPLC with superheated water. In addition, many details were improved and numerous references added. [Pg.427]

High-performance liquid chrottHtogtaphy (HPLC), definition. 3 HlUC, 217-223 HPLC, definition, 3 Hydrodynamic chromatography, 142 Hydrolytic stability, 107,346,369-370 Hydrophilic interaction (HlUC), 5,135,136, 217-223... [Pg.202]

Today, the most rational way to address the separation of very hydrophilic and uncharged compounds in HPLC is by utilizing hydrophilic interaction liquid chromatography (HILIC) [4-7], which is considered to be a variant of... [Pg.684]

McCalley, D.V. (2011) Hydrophilic interaction chromatography is it a viable complementary method to reversed-phase for the separation of polar or ionizable compounds Presentation TOl at HPLC 2011, Budapest, Hungary. [Pg.728]

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Hydrophilic interaction HPLC chromatography

Hydrophilic interactions

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