Hydrogen peroxide transducers

It is important to compare the catalytic properties of Prussian blue with known hydrogen peroxide transducers. Table 13.2 presents the catalytic parameters, which are of major importance for analytical chemistry selectivity and catalytic activity. It is seen that platinum, which is still considered as the universal transducer, possesses rather low catalytic activity in both H202 oxidation and reduction. Moreover, it is nearly impossible to measure hydrogen peroxide by its reduction on platinum, because the rate of oxygen reduction is ten times higher. The situation is drastically improved in case of enzyme peroxidase electrodes. However, the absolute records of both catalytic activity... 

Application of transition metal hexacyanoferrates for development of biosensors was first announced by our group in 1994 [118]. The goal was to substitute platinum as the most commonly used hydrogen peroxide transducer for Prussian blue-modified electrode. The enzyme glucose oxidase was immobilized on the top of the transducer in the polymer (Nation) membrane. The resulting biosensor showed advantageous characteristics of both sensitivity and selectivity in the presence of commonly tested reductants, such as ascorbate and paracetamol. 

Combined with the attractive performance of a Prussian blue-based hydrogen peroxide transducer, the proposed immobilization protocol provides elaboration of the most advantageous first-generation glucose biosensor concerning its sensitivity and detection limit. 

L.V. Lukachova, E.A. Kotel nikova, D. D Ottavi, E.A. Shkerin, E.E. Karyakina, D. Moscone, G. Palleschi, A. Curulli, and A.A. Karyakin, Electrosynthesis of poly-o-diaminobenzene on the Prussian Blue modified electrodes for improvement of hydrogen peroxide transducer characteristics. Bioelectrochemistry 55, 145-148 (2002). 

---