Prussian blue-based hydrogen peroxide transducer

Combined with the attractive performance of a Prussian blue-based hydrogen peroxide transducer, the proposed immobilization protocol provides elaboration of the most advantageous first-generation glucose biosensor concerning its sensitivity and detection limit. 

A.A. Karyakin and E.E. Karyakina, Prussian Blue-based artificial peroxidase as a transducer for hydrogen peroxide detection. Application to biosensors. Sens. Actuators, B B57, 268-273 (1999). 

Karyakin, A.A., E.E. Karyakina, and L. Gorton. 2000. Amperometric biosensor using Prussian blue-based artificial peroxidase as transducer for hydrogen peroxide. Anal. Chem. 72 1720-1723. 

The multilayer membrane coverage (of Fig. 6.6) improves the relative surface availability of oxygen and excludes potential interferences (common at the potentials used for detecting the peroxide product). Electrocatalytic transducers based on Prussian Blue layers (15) or metallized carbons (16), which preferentially accelerate the oxidation of hydrogen peroxide, are also useful for minimizing potential interferences. The enzymatic reaction can also be followed by monitoring the consumption of the oxygen cofactor. 

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