Hydrogen Peroxide Reagent

Dipping solution Acids Make up 1 ml 30% hydrogen peroxide (Perhydrol ) to 100 ml with water [1], 

Spray solution Thiabendazole Make up 3 ml 30% hydrogen peroxide to 100 ml with 10% acetic acid [2], 

Storage The reagent solutions should always be freshly made up. 

Many carboxylic acids are converted into fluorescent derivatives by oxidation and UV irradiation. The reaction mechanism has not been elucidated. 

The chromatograms are freed from mobile phase, immersed for 3 s in the reagent solution or homogeneously sprayed with it and then subjected to intense UV 

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More specifically, 2-aminophenol can be detected in solution using an iron(II) sulfate—hydrogen peroxide reagent (94). 3- Aminopheno1 has been analyzed colorimetrically by oxidation in base and subsequent extraction of a violet quinoneimide dye (95). A colorimetric method using... 

A rapid and clean oxidation of sulphides to sulphoxides can also be carried out using the titanium(III) trichloride/hydrogen peroxide reagent. On a milimole scale, the oxidation takes place in a time shorter than 20 min upon addition of a solution of hydrogen peroxide to a solution of the sulphide and titanium(III) trichloride in methanol at room temperature. It was suggested that the formation of a sulphoxide in this reaction resulted from a direct coupling of the hydroxy radical with cation radical 20 formed at the sulphur atom of the sulphide (equation 6). 

Although used predominantly with a,j5-unsaturated ketones thus far, the alkaline hydrogen peroxide reagent has also been found to react with., j3-unsaturated nitriles110 131Si 1117 and. more recently with 3-uiwattiratod aldehydes " 1311 and esters.1210 131 ... 

In general, the metal catalyst-hydrogen peroxide reagent is inferior to the corresponding metal catalyst-alkyl hydroperoxide systems for the epoxidation of olefins (see Section III.B.2). 

Peroxidase is an enzyme of the oxido-reductase class that reacts with a substrate containing hydrogen peroxide as the electron acceptor. To block this activity, a variety of hydrogen peroxide reagents can be applied to cells producing this enzyme. 

The oxidation of sulfides to sulfoxides or sulfones depend on the reaction conditions used. Hydrogen peroxide and ferf-butyl hydroperoxide have both been used as also has a variety of peroxycarboxylic acids. Since all of the peroxycarboxylic acids are stronger oxidants than hydrogen peroxide, reagents such as MCPBA have been used to oxidise sulfides to sulfoxides under very mild conditions. In the example shown in Eq. (38) the diastereomeric sulfoxides were obtained in good yield [46]. 

A) Decomposition with sulphuric acid/hydrogen peroxide Reagents... 

Hydrogen peroxide Reagent concentration Starting concentration 0.3% (0.02-3% if needed)... 

Hydrogen peroxide reagent. Dilute 25 cm of 20% v sulphuric acid solution and 4 ml 100 vol. hydrogen peroxide (Analar) to 100 cm with distilled water and shake. Allow to stand for 2 hours before use. Prepare this reagent fresh daily. 

Pipette 20 cm of the polyethylene extract into a 25 cm volumetric flask. Pipette in 2 cm ethanol and 2 cm hydrogen peroxide reagent and adjust the solution temperature to 25 + 3°C and make up to the 25 cm mark with ethanol and mix well. 

After 25 to 30 min fill a spectrophotometer cell with the test solution. Record the optical density at 5 min intervals until the maximum reading is obtained. The maximum optical density is usually obtained between 40 min and 120 min after addition of the hydrogen peroxide reagent depending on the concentration of Nonox in the test solution. 

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