Hydrogen peroxide, anhydrous phosphates

Peroxide Substrate Solution Dissolve 25.0 g of anhydrous dibasic sodium phosphate (Na2HP04), or 70.8 g of Na2H-P0412H20, in about 1500 mL of water, and adjust to pH 7.0 0.1 with 85% phosphoric acid. Cautiously add 100 mL of 30% hydrogen peroxide, dilute to 2000 mL in a graduate, and mix. Store in a clean amber bottle, loosely stoppered. The solution is stable for more than 1 week if kept at 5° in a full container. (With freshly prepared substrate, the blank will require about 16 mL of 0.250 N Sodium Thiosulfate. If the blank requires less than 14 mL, the substrate solution is unsuitable and should be prepared fresh again. The sample titration must be between 50% and 80% of that required for the blank.)... 

Bis(trimethylsilyl) peroxide, (CH3)3SiOOSi(CH3)3, is prepared from trimethylsilyl chloride, l,4-diaza[2,2,2]bicyclooctane, and Dabco s complex with 2 mol of hydrogen peroxide [127]. It is used alone [228] or in the presence of catalysts such as pyridinium dichromate [236] trimethylsilyl trifluoromethanesulfonate, CF3S03Si(CH3)3 [228, 237] or tris-(triphenylphosphine)ruthenium dichloride, [(C6H5)3P]3RuCl2 [236]. This reagent oxidizes primary alcohols to aldehydes (in preference to the oxidation of secondary alcohols to ketones [236]), ketones to esters or lactones Baeyer-Villiger reaction) [238], and nucleoside phosphites to phosphates [228]. All these oxidations require anhydrous conditions. 

The oxidation of phosphites to phosphates is very important in the chemistry of nucleoside phosphites, which must be handled under anhydrous conditions. For this purpose, anhydrous hydrogen peroxide bis(trimethylsilyl) peroxide, cumene hydroperoxide, and iV-methylmor ... 

LAPIS INFERNALIS (7761-88-8) A powerful oxidizer. Forms friction- and shock-sensitive compounds with many materials, including acetylene, anhydrous ammonia (produces compounds that are explosive when dry), 1,3-butadiyne, buten-3-yne, calcium carbide, dicopper acetylide. Contact with hydrogen peroxide causes violent decomposition to oxygen gas. Violent reaction with chlorine trifluoride, metal powders, nitrous acid, phosphonium iodide, red or yellow phosphorus, sulfur. Incompatible with acetylides, acrylonitrile, alcohols, alkalis, ammonium hydroxide, arsenic, arsenites, bromides, carbonates, carbon materials, chlorides, chlorosulfonic acid, cocaine chloride, hypophosphites, iodides, iodoform, magnesium, methyl acetylene, phosphates, phosphine, salts of antimony or iron, sodium salicylate, tannic acid, tartrates, thiocyanates. Attacks chemically active metals and some plastics, rubber, and coatings. 

Chemicals. (2-[N-Morpholino]ethanesulfonic acid) monohydrate (MES) and glucose oxidase (EC 1.1.3.4) type VII from Aspergillus niger (activity 1682 U/g solid) were purchased from Sigma Chemical Co. (St. Louis, MO). Sodium phosphate monobasic monohydrate, sodium phosphate dibasic anhydrous, citric acid monohydrate, acetonitrile (HPLC grade), tetrabutylammonium perchlorate (TBAP), glutaraldehyde (25% in water), and hydrogen peroxide (30% in water) were obtained from Fisher Scientific (Tustin, CA). Aniline, 1,2 phenylenediamine, and resorcinol were acquired from Aldrich (Milwaukee, WI). All the chemicals were used without purification. Double distilled ultrapure water was used for preparation of the buffers, standards, and electrochemistry work. 

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