Hydrazide biotinylation reagents

An analog of this biotinylation reagent with a longer spacer arm also exists. Biotin-LC-hydrazide contains a 6-aminocaproic acid extension off its valeric acid group (Thermo Fisher). 

Hydrazide-containing PEG-biotinylation reagents provide reactivity with carbonyl groups (e.g., aldehydes) to label carbohydrates or glycoproteins via hydrazone bond formation (Figures 18.19 and 18.20). The hydrazide group also may be coupled with carboxylate-containing... 

Figure 18.19 Biotin-PEG4-hydrazide is a hydrophilic biotinylation reagent that can be used to modify glycans or carbohydrates at their reducing end or after periodate oxidation to create aldehydes.

An analog of this biotinylation reagent with a longer spacer arm also exists. Biotin-LC-hydrazide contains a 6-aminocaproic acid extension off its valeric acid group (Pierce). The increased length of this spacer (24.7 A) provides more efficient interaction potential with avidin or streptavidin probes, possibly increasing the sensitivity of assay systems. The reactions of biotin-LC-hydrazide are identical to those of biotin-hydrazide. 

Buffer Bicarbonate or phosphate buffers are suitable for biotinylation. Organic buffers, sttch as Tris, which contain atnin.es, should be avoided, because they react with aminodabding reagents or interfere with the reaction between aldehydes and hydrazides However, HEPES and iiPPS, winch contain tertiary amines, are suit-... 

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