Hybrid filter media

Hybridization Procedures. Filters are placed in small leakproof vials, preincubated with a medium to block all adsorptive sites not occupied by DNA, then placed within reaction vials containing buffer and the labeled probe. On completion of the incubation, the filters are washed and dried, and the radioactivity is measured. 

Preincubate the filters in Denhardt s29 medium (0.02% bovine serum albumin, 2% polyvinylpyrrolidone, 0.02% Ficoll 400, in 2X SSC) for 30 min to 2 hr at the incubation temperature to be used for hybridization. Agitate the filters occasionally during preincubation. 

Bacterial cells are plated onto a solid medium that only allows the growth of transformed cells. Once the colonies become visible, the plate is blotted with a nitrocellulose filter. The cells clinging to the filter are lysed and the released DNA is denatured and deproteinized. After a labeled probe is added, unhybridized probe molecules are washed away. Cells that possess DNA sequences that hybridize with the probe are identified by comparing the autoradiogram of the filter with the master plate. 

Of the various groups shown two should be mentioned specifically, as they are somewhat anomalous. First of aU the screen is not a sedimentation system per se but is listed here since there is a good chance that a slurry which behaves as indicated would also be amenable to screening, in which case this may be a preferred method. Similarly, the inclined belt, being somewhat of a hybrid, could be considered by some to be a filter using its own solid mass as a medium. The same, of course would apply for inclined vibrators. 

The major advantage of the hybrid LTCC filter concept is the cost efficiency for small and medium volumes combined with a high scale of integration. Designs can be tailored to the specific application and produced on typical... 

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