Humanized transgenic mice

Backlund, J, Carlsen, S, Hdger, T, Hohn, B, Eugger, L, Kihlberg, J, Burkhardt, H, Holmdahl, R, Predominant selection of T cells specific for the glycosylated collagen t)fpe II epitope (263-270) in humanized transgenic mice and in rheumatoid arthritis, Proc. Natl. Acad. Sci. USA, 99,9960-9965, 2002. 

Stedman, C., Robertson, G., Coulter, S., and Liddle, C. (2004) Feed-forward regulation of bile acid detoxification by CYP3A4 studies in humanized transgenic mice. J. Biol. Chem. 279, 11336-11343. 

Chen Z, de Kauwe AL, Keech C, Wijburg O, Simpfendorfer K, Alexander WS, McCluskey J. Humanized transgenic mice expressing HLA DR4-DQ3 haplotype reconstitution of phenotype and HLA-restricted T-ceU responses. Tissne Antigens 2006 68 210-219. 

Display technologies add a very valuable dimension to the development of therapeutic antibodies derived from human Ig mice or to antibodies developed from synthetic libraries. They provide an alternative for developing therapeutic human antibodies for targets that are either identical or similar to the mouse homologues in humanized transgenic mice. 

DWNebert University of Cincinnati, Cincinnati, OH Utilize human transgenic mouse lines to define the precise role of the human Ah receptor in studies of toxicity and cancer caused by 2,3,7,8-TCDD and other environmental chemicals... 

Cheung, C., Yu, A. M., Ward, J. M., Krausz, K. W., Akiyama, T. E., Feigenbaum, L., and Gonzalez, F. J. (2005). The cyp2el-humanized transgenic mouse role of cyp2el in acetaminophen hepatotox-icity. Drug Metab Dispos 33, 449-457. 

Cheung C, Yu AM, Ward JM, Krausz KW, Akiyama TE, Feigenbaum L, Gonzalez FJ. The CYP2E1-humanized transgenic mouse Role of CYP2E1 in acetaminophen hepatotoxicity. Drug Metab Dispos 2005 33 449-457. 

In a humanized transgenic mouse model, P450 2C18 was expressed in liver and kidney in a male-specific manner [801], but the relevance to humans is unknown. 

Guo HG, Sadowska M, Reid W, Tschachler E, Hayward G, Reitz M. Kaposi s sarcoma-like tumors in a human herpesvirus 8 ORF74 transgenic mouse. J Virol 2003 77(4) 2631-2639. 

Catalyzes 6-hydroxlation of melatonin in vitro and in transgenic mouse brain (Ma et al., 2005). 0.0017 CYPIBI vnRNAJGAPDH mRNA in whole human brain by RT-RT-PCR (Nishimura et al., 2003). 

A further advance in antibody technology is the development of transgenic mouse human strains. XenoMouse animals have been engineered in such a way that they now produce exclusively human antibodies rather than murine antibodies when immunized. The use of XenoMouse animals to produce MAbs avoids the need for any engineering of the antibody genes, since the products are already 100% human protein. XenoMouse animals are fully compatible with standard hybridoma technology and can be readily adopted by laboratories experienced in monoclonal antibody production [56]. 

Lonberg N Fully human antibodies from transgenic mouse and phage display platforms. Curr Opin Immunol 2008 20 450. [PMID 18606226]... 

A new transgenic mouse caiiying a gene for human Alzheimer s disease developed. Games et al Nature, 373. 476-477, 523-537 (199.5)... 

Shimizu, A., Nussenzweig, M.C., Mizuta, T.-R., Leder, P., Honjo, T. (1989). Immunoglobulin double-isotype expression by trans-mRNA in a human immunoglobulin transgenic mouse. Proc. Natl. Acad. Sci. USA 86, 8020-8023. 

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