Human papillomavirus E7 protein

Similar to two-hybrid screens, SPRi can be used to detect conditions under which proteins do not interact. The interaction of human papillomavirus E7 protein and retinoblastoma tumor suppressor protein RB was studied by Jung and co-workers [49]. Fifteen hundred protein spots containing immobilized E7 protein were produced, and an interaction with added RB protein was detected using SPR. The addition of a peptide derived from a motif on E7 along with RB has been observed to clearly disrupt the interaction through lack of an SPR signal. The one peptide discussed in this study can easily be replaced with an array of... 

Li, Y., Li, X., liu, H., et al. (2014) Intranasal immunization with recombinant lactococci carrying human papillomavirus E7 protein and mouse interleukin-12 DNA induces E7-specific antitumor effects in C57BL/6 mice. Oncol Lett 7, 576-582. 

Munger, K., Wemess, B.A., Dyson, N., et al. (1989) Complex formation of human papillomavirus E7 proteins with the retinoblastoma mmor suppressor gene product. EMBO J 8, 4099-4105. 

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