Human hypoxanthine-guanine phosphoribosyl transferase

Single dose cynomolgus monkeys Repeat dose mice, rats, cynomolgus monkeys Fertility and early embryonic development rats Developmental rats, mice, rabbits Prenatal and postnatal development rats Genetic toxicology Ames reverse mutation, Chinese hamster ovary hypoxanthine guanine phosphoribosyl-transferase forward point mutation assay, cytogenetics in human lymphocytes... 

F7. Fujimoto, W. Y., and Seegmiller, ). E., Hypoxanthine guanine phosphoribosyl-transferase defidency activity in normal mutant and heterozygote cultured human sldn fibroblasts. Proc. Nat. Acad. Set. U.S. 65, 577 (1970). 

J.E. Biochemical bases of accelerated purine biosynthesis de novo in human fibroblasts lacking hypoxanthine-guanine phosphoribosyl-transferase. J.Biol.Chem. 243, 1166, 1968. 

A. A. van Zeeland, Y. C. E. M. de Ruijfer, and J. W. I. M. Simons, The role of 8-azagua-nine in the selection from human diploid cells of mutants deficient in hypoxanthine-guanine-phosphoribosyl-transferase (HGPRT), Mutat. Res. 23, 55 (1974). 

Several mammalian cell systems have been developed in order to determine the potential of food additives and other environmental chemicals to cause gene locus mutations. Some employ rodent cells, as described by Clive and Spector , whereas others use diploid human lymphoblasts as indicator organisms The human lymphoblast system measures the response of hypoxanthine guanine phosphoribosyl transferase (hgprt). Considerable care must be used with these systems for example, the importance of phenotypic lag has been clearly demonstrated by Penman and Thilly. ... 

STABILIZATION BY PRPP OF CELLULAR PURINE PHOSPHORIBOSYL-TRANSFERASES AGAINST INACTIVATION BY FREEZING AND THA ING. STUDY OF NORMAL AND HYPOXANTHINE-GUANINE PHOSPHORIBOSYL-TRANSFERASE DEFICIENT HUMAN FIBROBLASTS... 

Keough, D. T., Ng, A. L., Winzor, D. J., Emmerson, B. T., and de Jersey, J. (1999). Purification and characterization of Plasmodium falciparum hypoxanthine-guanine-xan thine phosphoribosyl-transferase and comparison with the human enzyme. Mol. Biochem. Parasitol. 98,29-41. 

Cultured human skin fibroblasts are commonly utilized in the detection of hemizygosity and heterozygosity for hypoxanthine-guanine phosphoribosyltrans-ferase (HGPRT) deficiency. Two obstacles are encountered in the determination of HGPRT and adenine phosphoribosyl-transferase (APRT) in extracts of cultured skin fibroblasts the sensitivity of these enzymes in dilute cell suspension to freezing and thawing (l), and the presence of nucleotidase activity (2). 

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