Human Fab fragments

Barbas, C F, Bjorling, E., Chiodi, F., Dunlop, N., Cababa, D., Jones, T M., Zebedee, S. L., Persson, M A. A, Nara, P. L, Norrby, E., and Burton, D. R. (1992) Recombinant human Fab fragments neutralise human type-1 immunodeficiency virus m vitro Proc Natl Acad Sci USA 89, 9339-9343... 

De Haardt, H. J., van Neer, N., Reurst, A., Hufton, S. E., Roovers, R. C., Henderikx, P., et al. (1999) A large non-immunized human fab fragment phage library that permits rapid isolation and kinetic analysis of high affinity antibodies. J. Biol. Chem. 274, 18,218-18,230. 

CN immunoglobulin G (human-mouse monoclonal c7E3 clone p7E3VHhCy4 Fab fragment antihuman... 

In chimpanzees, administration of Fab fragments of a monoclonal anti-F-VII antibody preceding an endotoxin bolus injection effectively blocked the activation of the coagulation pathway (B25). Administration of monoclonal anti-lL-6 under the same experimental conditions attenuated the activation of coagulation, while the fibrinolytic system remained unaltered. However, administration of monoclonal anti-TNF enhanced the tendency to microvascular thrombosis (P17,18). Monoclonal anti-TF antibodies administered to baboons as a pretreatment attenuated coagulopathy after induction of E. coli sepsis in these animals (T4). Primates pretreated with anti-C5a antibodies before infusion of E. coli developed less hypotension and had better survival rates than untreated animals, who developed ARDS and septic shock with a mortality rate of 75% (S35, Z6). No favorable treatment results have been published yet with one of these treatment modalities given to humans. 

SIAB and sulfo-SIAB have been used to make a high-capacity RNA affinity column for the purification of human IRP1 and IRP2 (Allerson et al., 2003), to couple antibodies or Fab fragments to amine-modified microparticles (Harma et al., 2000), and in the attachment of oligonucleotides to surfaces for detection arrays (Adessi et al., 2000). 

Motta-Hennessy, C., Eccles, S.A., Dean, C., and Coghlan, G. (1985) Preparation of 67Ga-labeled human IgG and its Fab fragments using deferoxamine as chelating agent. Eur. J. Nucl. Med. 11, 240-245. 

Plasmid DNA (pUC18) Fab fragment from an IgG purified from human sera pH 7.5,30°C Nicked DNA Autoantibodies Human serum IgG fraction (Fab) was found to hydrolyse DNA. Unknown immunogen 4.3 x 101 1.4 x 101 nr 6.5... 

A reversible, direct fluoroimmunosensor for human serum albumin (HS A) measurement has been described by Bright et al.(m> Antibody Fab fragments are first immobilized on small quartz plates by hinge-region thiols, and then dansylated. The immunosensor is formed by attaching the quartz plates with bound Fab to the distal end of a bifurcated fiber-optic probe, which transmits both the excitation and emission. Binding of ffSA to the immunosensor results in a three- to five-fold enhancement of dansyl fluorescence. The sensor can be reused up to 50 times, with a detection limit of about 1.8 x 10-8 M, and a somewhat limited dynamic range. 

Kelley, R.F., M.P O Connell, P. Carter, L. Presta, C. Eigenbrot, M. Covarruabias, B. Snedecor, R. Speckart, G. Blank, D. Vetterlein, and C. Kotts. 1993. Characterization of humanized anti-pl85HER2 antibody fab fragments produced in Escherichia coli. ACS Symp Ser 526 218-239. 

Sapra P, Moase EH, Ma J, et al. Improved therapeutic responses in a xenograft model of human B lymphoma (Namalwa) for liposomal vincristine versus liposomal doxorubicin targeted via anti-CD19 IgG2a or Fab fragments. Clin Cancer Res 2004 10 1100. 

Jacobo-MoUna, A., Qark, A. D., Jr., Williams, R. L., Nanni, R. G., Clark, P, Ferris, A. L., Hughes, S. H. and Arnold, E. (1991). Crystals of a ternary complex of human immunodeficiency virus type 1 reverse transcriptase with a monoclonal antibody Fab fragment and double-stranded DNA diffract X-rays to 3.5- A resolution. Proc. Natl. Acad. Sci. USA 88,10895-10899. 

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