Human corneal epithelial cell lines

Araki-Sasaki K, Ohashi Y, Sasabe T, Hayashi K, Watanabe H, Tano Y, Handa H. An SV40-immortalized human corneal epithelial cell line and its characterization. Invest Ophthalmol Vis Sci 36 614—621 (1995). 

Kruszewski FH, Walker TL, DiPasquale LC. Evaluation of a human corneal epithelial cell line as an in vitro model for assessing ocular irritation. Fundam Appl Toxicol 36 130-140 (1997). 

Saarinen-Savolainen, P., et al. 1998. Evaluation of cytotoxicity of various ophthalmic drugs, eye drop excipients and cyclodextrins in an immortalized human corneal epithelial cell line. Pharm Res 15 1275. 

Burgalassi, S., et al. 2001. Cytotoxicity of potential ocular permeation enhancers evaluated on rabbit and human corneal epithelial cell lines. Toxicol Lett 122 1. 

Kruszewski, F. H Walker, T. L., and DiPasqualc. L, C. (1997), Evaluation of human corneal epithelial cell line as an i>i viim model for assessing ocular irritancy, Fundam, Appl. Toxical 36, 130-160. 

The HCE-T Tissue Construct (Gillette model) uses a transfected human corneal epithelial cell line (Kahn et al., 1993) cultured on collagen-membrane cell culture inserts, which, at the air-liquid interface, stratify to form a four- to six-layer epithelium, known as the HCE-T model. Transepithelial permeabiUty (TEP) to sodium fluorescein and transepitheUal electrical resistance (TER) have been identified as physiologically relevant parameters to evaluate the barrier function of the corneal epithelium. Cell viability can be determined by the MTT assay, and histomorphology can also be used as an endpoint (Kruszewski et al., 1997). 

The HCE-T model was established in 1995 by Araki-Sasaki and coworkers [56], It was formed by transfection of human corneal epithelial cells from a 47-year-old female donor with a recombinant SV40-adenovirus, which lacked the origin of viral replication (i.e., not able to produce viral particles). The cell line has good growth characteristics and shows a cobblestone-like appearance. The cell line is intensively used by Urtti and coworkers and represents a standard... 

The Clonetics cell line from Cambrex Bio Science represents a cultured human corneal epithelial model (order no. CMS-2015 Cambrex Bio Science, Walkersville, MD). The culture model was generated by isolation of cells from normal human corneal tissues. The cells were then infected with an amphotropic recombinant retrovirus containing HPV-16 E6/E7 genes to extend the useful cell life span. The Clonetics cell model is a very recent entry into the immortalized corneal cell line field, but it has been proved to be useful for toxicity testing as well as in vitro drug permeation studies so far. Because of its very recent introduction, further examinations have to be undertaken to... 

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