HT29-MTX

Pontier, C. Pachot, J. Botham, R. Lefant, B. Amaud, R, HT29-MTX and Caco-2/TC7 monolayers as predictive models for human intestinal absorption Role of mucus layer, J. Pharm. Sci. 90, 1608-1619 (2001). 

Walter,E. Janich, S. Roessler, B.J. Hilfinger,J.M. Amidon, G. L., HT29-MTX/Caco-2 cocultures as an in vitro model for the intestinal epithelium In vitro-in vivo correlation with permeability data from rats and humans, J. Pharm. Sci. 85, 1070-1076 (1996). 

Hilgendorf, C. Spahn-Langguth,H. Regardh,C. G. Lipka, E. Amidon,G. L. Langguth, P., Caco-2 vs Caco-2/HT29-MTX co-cultured cell lines Permeabilities via diffusion, inside- and outside-directed carrier-mediated transport, J. Pharm. Sci. 89, 63-75 (2000). 

Behrens, I., Stenberg, P., Arturs-son, P., Kissel, T., Transport of lipophilic drug molecules in a new mucus-secreting cell culture model based on HT29-MTX cells, Pharm. 

Carriere V, Lesuffleur T, Barbat A, Rousset M, Dussaulx E, Costet P, de Waziers I, Beaune P, Zweibaum A (1994) Expression of cytochrome P-450 3A in HT29-MTX and Caco-2 clone TC7. FEBS Lett 355 247-250. 

When using Caco-2 or TC7 cells, parameters such as transepithelial electrical resistance (TEER), differentiation marker, morphology, P-gp expression, and monolayer integrity need to be tested failure to do so is often the reason for wide interlaboratory variations. Caco-2 cell lines contain only a single cell type and do not form a mucus layer. As this can limit the absorption of lipophilic compounds in particular, the coculturing of Caco-2 cells and mucus-secreting lines such as HT29-MTX has been proposed to overcome this limitation [48]. 

Reduction of the disulfide bond in the thiol-rich environment, resulting in the release of the entrapped protein, was also the working principle of another intracellular protein delivery system. The DDS was based on linear polyamidoamines (PAAs) that formed a PEC with negatively charged human serum albumin (HSA). The PEC showed mucoadhesive properties and released the immobilized HSA under intracellular conditions due to the cleavage of the disulfide bonds, while the complex was stable under extracellular conditions. This resulted in enhanced uptake by exposed human-derived intestinal Caco-2/TC7 cells and HT29-MTX mucus/ secreting cells. 

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