HPLC high performance liquid column packing

A more sophisticated method of column chromatography is known as HPLC, high-performance liquid chromatography. This employs very fine solid particles which pack closely together. This increases the surface available for adsorption, and so improves the separation, but because the solid particles are packed tightly together, a pump is needed to force the mobile liquid phase through the column. 

Reversed-phase high-performance liquid chromatography (HPLC) column 50 mm x 3.2-mm i.d. with Kromasil 5- um Gig packing High-performance liquid chromatograph coupled to a triple-quadrupole mass spectrometer with an atmospheric pressure chemical ionization (APCI) source Gel permeation chromatograph with a 60 mm x 25-mm i.d. column packed with Bio-Beads SX-3 (50-g)... 

Particulate sorbents are available almost exclusively in the shape of micrometersized beads. These beads are packed in columns and represent currently the most common stationary phases for high-performance liquid chromatography (HPLC). Despite their immense popularity, slow diffusional mass transfer of macromolecular solutes into the stagnant pool of the mobile phase present in the pores of the separation medium and the large void volume between the packed particles are considered to be major problems in the HPLC of macromolecules, frequently impairing their rapid and efficient separation [1]. 

High-Performance Liquid Chromatography. A Varian 5060 delivery system was used for this work with detection by UV absorption. Either a Varian UV-50 variable wavelength detector or a Hewlett Packard 1040A scanning diode array detector was used. All HPLC columns were packed in our laboratory (10) with 5-/um particle size Spherisorb-ODS, Spherisorb-CN (Phase Separations), or 8-pm particle size Zorbax-CN (Dupont Ltd). HPLC columns (20 or 25 cm X 4.6 mm i.d.) were coupled via short lengths of stainless steel capillary tubing (5 cm X 0.25 mm i.d.). Separation conditions were as follows ... 

High-performance liquid chromatography (HPLC) is a fast technique that, with high precision and specificity, separates mixtures into individual ingredients. Used as a routine procedure, it has several advantages It can be completely automated sample cleanup and preparation are simple and the reproducibility of the packing material means that the analytical conditions remain the same for a new column. 

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