HPCE high performance capillary

HPCE High-performance capillary (2) Interaction chromatography... 

PLT = palytoxin, OST = ostreocins, LC-MS = Liquid Chromatography-Mass Spectrometry, HPLC = high performance liquid chromatography, HPCE = high performance capillary electrophoresis. 

A high performance capillary electrophoresis (HPCE) was described for the separation and simultaneous determination of OTC, TC, CTC, DC, and chloramphenicol in honey. The use of buffer pH 3.2 containing 0.02 mol/L Na2HP04 and 0.01 mol/L citric acid with addition of 4% (v/v) A-methylmorpholine and 12% (v/v) acetonitrile demonstrated a good separation of these five antibiotics within 20 min. The proposed method gave detection limit (signal to noise ratio > 5) of 20 pg/L for OTC [26],... 

A further improvement of the more traditional slab gel analysis is the use of high-performance capillary electrophoresis (HPCE), which combines the separation power of high-performance liquid chromatography (HPLC) with the selectivity and speed of conventional gel electrophoresis. However, as HPCE separations are often performed using fused silica capillaries the positively charged histone molecules... 

Shibukawa et al. (40) discussed the frontal analysis method, also called high-performance frontal analysis (HPFA) or high-performance capillary electrophoresis/frontal analysis (HPCE/FA), compared it to conventional methods, and focused on the application to stereoselective protein binding. The affinity of the drugs warfarin, verapamil, and carbamazepine and the drug candidate BOF-4272 to HSA was investigated. 

The reader is directed to Ref. 5, which makes an interesting comparison between HPLC and other analytical methodologies for the determination of carbohydrates in foods. Additionally, notable progress has been made in the application of high-performance capillary electrophoresis (HPCE) in this field (8-11). However, given the scope of this chapter, we will focus on the advantages and drawbacks of other chromatographic techniques versus HPLC. 

D. Hoegger and R. Freitag, HPCE 2000, 13th International Symposium on High Performance Capillary Electrophoresis and Related Microscale Techniques, Saarbrticken, Germany, February 20-24, 2000, Abstracts p.230. 

In this book, the focus is on the use of high performance liquid chromatography (HPLC) for the separation and detection of biochemical components produced from enzymatic reactions. But the purpose of the book is also to introduce HPLC and high performance capillary electrophoresis (HPCE) as methods applicable for separation and detection of a variety of components, even those not produced by enzymatic reactions. 

Using these criteria as yardsticks, the first edition has been a success. It is now obsolete, however Because of the increases in both number and types of activities assayed, it is no longer an accurate catalog of enzymatic activities investigated by means of the HPLC method. For this work to continue to serve as a reference source, it would need updating. While it was the obsolescence of the first edition that in part prompted the development of a second edition, there were other considerations as well. These included the introduction of high performance capillary electrophoresis (HPCE) as a method for separation, the development of microdialysis as a method for collection of samples... 

Most forms of detection in High-Performance Capillary Electrophoresis (HPCE) employ on-capillary detection. Exceptions are techniques that use a sheath flow such as laser-induced fluorescence [1] and electrospray ionization mass spectrometry [2],... 

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