How to Pick an HCS System

The decision of which HCS system to purchase should really be dominated by comparing the trade-offs among the ability of the software to interpret image features as relevant biological events, the time required to develop an assay, the optical resolution, the throughput, and the balance of cost/reliability/service. It is impossible to optimize for all of these axes simultaneously, and therefore, different systems have prioritized different features. 

Almost all HCS assays will require at least two fluorescent dyes. One is often to identify which objects are cells or nuclei and a second is to look for specific subcellular expression or localization of a protein or morphological change. A third dye can be used for additional information, such as cell type, co-localization of a second protein, etc. Systems that simultaneously measure two or more wavelengths are advantageous not only for speed but also to enable live cell assays and the measurement of kinetic response to the addition of a reagent. Environmental control is sometimes required for these live-cell assays. Most higher throughput applications are done with fixed cells for convenience. 

There are many other factors that should be considered when picking an HCS system. In particular, software ease of use is paramount. When assessing ease of use, consider the ease of assay development, the flexibility of the picking cell populations and responses, whether the software architecture is open or closed, and the availability of 

Given factors discnssed above, the choice of HCS system shonld probably be driven by software qnality, then resolution and speed. Once the list is narrowed, qnality of service, data storage facility, cost, ability to be automated, and reliability need to be factored into the final decision. 

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