How to Improve the Analysis of DNA by MALDI

The optimisation of the MALDl process consists of identifying the right matrix and preparation method for an analyte. How matrices function in MALDl is not well understood. The chemical structure of DNA is complex and its interaction with a matrix during the desorption/ionisation process eludes investigation. Only empirical findings progressed the method. It was observed that DNA analysis by MALDl was very inefficient [15], for example 100 times more DNA has to be used in a preparation to achieve a similar signal intensity comparable to peptides. [Pg.52]

There are two common matrix preparation methods, thin-layer and dried droplet preparation. For thin-layer preparations the matrix is tqiplied to the MALDl target plate in a volatile solvent, such as acetone. The solvent spreads and evaporates [Pg.52]

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