Hosts plant transformation

Fig. 1. Infection and transformation of a suitable host plant by Agrobacterium tumefaciens (from Bryant, 1988).

Fig. 2. Schematic representation of the Ti-plasmid of Agrobacterium, showing the genes involved in transformation of host plants. Note that the genetic map is not to scale in reality, the T-region makes up about 10% of the total plasmid genome (from Bryant, 1988).

In nature, most plant roots are invaded by fungi and transformed into mycorrhizae or "fungus roots" (25). The host plant and fungus form a symbiotic relationship whereby nutrients absorbed from the soil by the fungus are released into the host cell and the mycorrhizal fungus obtains nutrients from the host. Mycorrhiza formation is complex and depends on the dynamic interaction of the host plant, fungus and soil. Once formed, mycorrhizae have a profound influence on growth and development of the host plant (26-28). 

Method of Transformation and Selection of Host Plant Species... 

Mikkelsen L, Roulund N, Liibeck M, Funck Jensen D. The perennial ryegrass endophyte Neotyphodium lolii genetically transformed with the green fluorescent protein gene (gfp) and visualization in the host plant. Mycol Res 105 644-650, 2001. 

Plant transformation is a complex process that depends on a number of various factors. The activity of the host DNA repair machinery represents one of the key factors that influence transgene integration efficiency. In our study, we demonstrated that high activity of HR at the time of transformation can significantly improve the STF. We suggest that the modification of a plant growth medium, using factors that affect... 

Three studies of the transformation of host-plant monoterpene... 

Specialized lepidopterans not only sequester PAs from their host plants but also transform them into insect-specific metabolites. The two best-studied examples are the PA-derived pheromones and the so-called insect PAs. 

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