Host cells large-scale cultivation

Large-Scale Cultivation of Host Cells for Production of Recombinant Proteins... 

Human diploid cell lines have been utilized classically as in vitro hosts for the propagation of polio, mumps, rubella, cytomegalovirus, varicella-zoster, rabies, hepatitis A, respiratory syncytial virus, parainfluenza and many other viruses. Other uses in the biotechnology industry include large-scale cultivation for the production of various cellular products, such as human interferon beta. 

Recently, plant cells have also been considered to be an alternative host for the production of recombinant proteins since they are able to glycosylate proteins [133, 139]. Of the various systems used for cultivation, such as hairy roots, immobilized cells, and free cell suspensions, the latter is regarded to be most suitable for large-scale applications. Full-size antibodies, antibody fragments, and fusion proteins can be expressed in transgenic-plantcell systems, such as Nicotiana tahacum, pea, wheat, and rice using shake-flask or fermentation cultures [136]. Yet, these systems are still of low commercial importance due to their unadvantageous productivity. 

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