Horizontal chambers gradient development

Chapter 4 discusses the selection and optimization of mobile phases for successful separations in PLC. Chapter 5 details procedures for sample application and development of layers, and Chapter 6 complements Chapter 5 by dealing specifically with the use of horizontal chambers for the development of preparative layers, including linear, continuous, two-dimensional, gradient, circular, and anticircular modes. 

FIGURE 6.10 The cover of the prepared chromatoplate for gradient development in a fully online horizontal chamber 1 — solvent system inlet, 2 — Silcoflon cover sheet, 3 — chromatoplate, 4 — solvent system outlet, 5 — channel for solvent system. (From Nyiredy, Sz. and Benko, A., Proceedings of the International Symposium on Planar Separations, Planar Chromatography 2004, Nyiredy, Sz., Ed., Research Institute for Medicinal Plants, Budakalasz, 2004, pp. 55-60. With permission.)... 

The horizontal DS chamber has been described for use in isocratic, gradient, and multiple development, as well as simultaneous development using different mobile phases under different conditions (Waksmundzka-Hajnos and Wawrzynowicz, 1994 Matysik, 1994 Matysik and Wojtasik, 1994 Matysik et al., 1994 Golkiewicz, 1996 Matysik and Toczolowski, 1997). A modified version of the DS chamber allows the solvent entry position to be changed by movement of the plate, increasing efficiency in single and multiple development (Dzido et al., 1995). 

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